wittenburg/hsrecombi
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AUTHORS: D. Wittenburg, N. Melzer DATE: June 7, 2023 OBJECTIVE: Create a breed-specific genetic map from genotypes of half-siblings and interactively visualise the outcome SOURCE: The R package CLARITY is available at https://github.com/nmelzer/CLARITY An online version of CLARITY is available at https://nmelzer.shinyapps.io/clarity/ INSTRUCTIONS: 1. The pipeline for creating a genetic map (available in subfolder makefile_for_<real/sim>_data) requires make version >= 4.3; this version allows for grouped targeting. Ensure that required R packages have been installed (hsrecombi >=1.0.0, ggplot2; optional: AlphaSimR), then call "make all". For each chromosome i, marker names, genetic and physical positions are stored as a list in geneticpositions_chr<i>.RData. Further interim results are available. 2. To prepare input data for the R-Shiny app CLARITY version >= 1.0.0 in the required format, run "prepare_data_rshiny.R" in the same working directory as "make all" (requires R packages magrittr, hsrecombi). The output Rdata will then be content of folder CLARITY/inst/extdata. Note that, unless working with nchr = 29 bovine autosomes, modifications to the app become necessary. (3.) You may check the Rdata format by running "description_data_rshiny.Rmd" and comparing with https://github.com/nmelzer/CLARITY/blob/main/description_data_rshiny.html.
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Estimation of Recombination Rate and Maternal LD in Half-Sibs
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