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The MiHAD is an immunoinformatics database of minor histocompatibility antigens. It includes comprehensive information about experimentally validated MiHAs and also includes the predicted MiHAs. Initially, we developed a pipeline to extract the differential missense mutations from 205 donor-recipient paired whole genome sequencing data, then predicted their binding affinity with HLA molecules.

MiHAD overview:
![](https://github.com/wwang-nmdp/MiHAD/blob/master/images/DesignOfMiHAD.png)

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