It is a challenge to classify protein-coding or non-coding transcripts, especially those re-constructed from high-throughput sequencing data of poorly annotated species. We developed and evaluated a powerful signature tool, Coding-Non-Coding Index (CNCI), by profiling adjoining nucleotide triplets to effectively distinguish protein-coding and non-coding sequences independent of known annotations. CNCI is effective for classifying incomplete transcripts and sense-antisense pairs. The implementation of CNCI offered highly accurate classification of transcripts assembled from whole-transcriptome sequencing data in a cross-species manner, that demonstrated gene evolutionary divergence between vertebrates, and invertebrates, or between plants, and provided a long non-coding RNA catalog of orangutan.
Release : CNCI version 2 Feb 28, 2014
CNCI has update to version 2,in this version some bugs have be fixed,and more friendly for users. CNCI can run in 32-bit Linux, 64-bit Linux. Please note that: CNCI's input file must not be empty.
At the first time to running CNCI, we suggest you to install "libsvm-3.0" that stored in our package.
git clone firstname.lastname@example.org:www-bioinfo-org/CNCI.git cd CNCI unzip libsvm-3.0.zip cd libsvm-3.0 make cd ..
HELP for CNCI subroutines
compare.py: compare the merged/assembled transcripts with known gene annotation!
Usage: compare.py [-h] -c coding_ref -n noncoding_ref -i input_gtf -o out_dir
-h, --help show this help message and exit.
-c CODING_REF, --coding_ref=CODING_REF
(Required.) The path of coding reference gtf file. Two mandatory attributes (gene_id "value"; transcript_id "value") should be provided in the file. Some files which has been prepared could be download at http://www.bioinfo.org/np/
-n NONCODING_REF, --noncoding_ref=NONCODING_REF.
(Required.) The path of lincRNA reference gtf file. Two mandatory attributes (gene_id "value"; transcript_id "value") should be provided in the file. Some files which has been prepared could be download at http://www.bioinfo.org/np/
-i INPUT_GTF, --input_gtf=INPUT_GTF
(Required.) The path of user input assemble gtf file. This file usually be generated by cufflinks/cuffcompare/cuffmerge. Also, two mandatory attributes (gene_id "value"; transcript_id "value") should be provided in the file.
-o OUT_DIR, --out_dir=OUT_DIR
(Required.) Output dirctory of the results.
CNCI.py: A classification tool for identify coding or non-coding transcripts (fasta files and gtf files)
-f or --file : input files
-o or --out : assign your output file in current directory (this parameter will produce a Temp sub-folder in current directory, and will remove it automatically at the end of programming), and the result is stored in xxx.index
-p or --parallel : assign the running CUP numbers
-m or --model : assign the classification models ("ve" for vertebrate species, "pl" for plat species)
-g or --gtf : if you input files is gtf format please use this parameter
-d or --directory : if you use the -g or --gtf this parameter must be assigned, within this parameter please assign the path of your reference genome.
filter_novel_lincRNA.py: A tool that can convert the index file which produced by python CNCI_package/CNCI.py to four gene classes (novel_lincRNA,novel_coding, ambiguous_genes and filter_out_noncoding)
Usage: filter_novel_lincRNA.py [-h] [-s 0] [-l 200] [-e 2] -i cnci_index -g unannotated_gtf -o out_dir
-h, --help show this help message and exit
-i INDEX, --index=INDEX
(Required.) The path of coding/noncoding index file. This file is the output file of CNCI.py.
-g GTF, --gtf=GTF
(Required.) The path of potentially_novel gtf file. This file could be generated by compare.py.
-s SCORE, --score=SCORE
(Optional.) Threoshold of CNCI score. RNAs with score less than SCORE will be classified as noncoding. The Default is 0 .
-l LENGTH, --length=LENGTH
(Optional.) Minimal length of lincRNA. lincRNA with length >= LENGTH will be kept. The Default is 200.
-e EXON_NUM, --exon_num=EXON_NUM
(Optional.) Minimal exon number of lincRNA. lincRNA with exon number >= EXON_NUM will be kept. The Default is 2.
-o OUT_DIR, --out_dir=OUT_DIR
(Requried.) Output directory of the results.
you can use CNCI subroutines like our example:
python CNCI_package/CNCI.py -f unannotation.gtf -g -o test -m ve -p 8 -d hg19.2bit python filter_novel_lincRNA.py -i test.index -g unannotation.gtf -s 0 -l 200 -e exon_num -o out_dir python extract.py -i novel-noncoding.gtf,nov.gtf -n known-non-coding.gtf -c known-coding.gtf
Please note that : "libsvm-3.0 must be installed accordance with our instruction in SETUP section"
- Liang Sun, Haitao Luo, Dechao Bu, Guoguang Zhao, Kuntao Yu, Changhai Zhang, Yuanning Liu, RunSheng Chen and Yi Zhao* Utilizing sequence intrinsic composition to classify protein-coding and long non-coding transcripts. Nucleic Acids Research (2013), doi: 10.1093/nar/gkt646
- Yi Zhao : email@example.com