Confusing “-t” parameter in stage two

[Wang0293]

“-t” parameter lose efficacy in stage two sometimes , for example:
I used "-t 8" parameter in stage two, and info shows that:

INFO: BLAST settings: 21401791 bps, 232757 reads, 8 threads, mt_mode 0.

And only 1 threads used.
Obviously, the mt_mode is setted incorrectly.
Do you know the possible cause?

[xinehc]

Hi,

this is because blast requires at least 2500000 bp per thread to enable mt_mode 1.

    mt_mode = '1' if nbps / self.thread >= 2500000 else '0'

In your case the number of bp is small, so blast should not take a long time to finish even with mt_mode 0.

[Wang0293]

Very thanks for your patience to my stupid😣 question.