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Regarding error related to fastq files #64
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Your fastq file is truncated/incomplete somehow. Did you by any chance concatenated a fastq with a fasta file? The + line is missing in line 29480427, please double check the file. |
I would recommand to run Reference: https://bioinf.shenwei.me/seqkit/usage/#sana |
Did you check file <input/HeP-1057-162.fa>? Although its named after .fa, |
Yup, earlier I changed the extension of fq.gz files after extraction to .fa. But then I run the pipeline on .fq.gz files only (the ones I got after quality check, adaptor trimming and removal of host sequences). I am getting the same error. Yes, all the files are fastq files in gzip format. And all the files are giving this same error. How to know if the files are truncated. The de novo assembled files of these fastq are just fine. Everything in the downstream analysis is just fine. I don't know why it is giving this error. |
try |
The file after seqkit fq2fa is not gzipped, you need to remove .gz otherwise it will be mistakenly considered as a gzipped file.
Please try not to manually editing the metadata file. It may lead to unexpected results.
I am actually not sure what is wrong with your input file. If you don't mind please attach a minimal reproducible example file so that I could check. |
Is it sufficient. Please let me know. Also, Why is that, same file when run on older version of ARGS-OAP (on Desktop) is giving result but when run on recent version (On server) is not giving the result. |
If you remove .gz then it should work:
Your files are not gziped. You can simply check whether a file is gzipped using |
Hello @xinehc ,
Also,
I can't really understand what is wrong. I attach a file of mine as proxy. Thanks in advance https://drive.google.com/file/d/1y-20f7rUJX2cm3rYBIdmxPtdI-iq6qec/view?usp=drive_link |
My issue was resolved. I tried everything but actually this was the error. There was nothing wrong with the sequences. Also, the same command runs on the local system with the same file that was giving the error on the server. |
@chanchalrana thank a lot for your feedback, I will try to adopt same procedure! My problem is only that I also have to deinterleave my files, as they are paired ends while your were not, if I understood correctly. UPDATE: I was not able to run the args_oap on the server, but worked on my laptop. Thanks @chanchalrana for the feedback. |
Hello!!
I am running args_oap on fastq files and in stage one I am getting the error as:
Kindly tell me the solution for this. I am getting it for all the files.
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