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prepare_transcripts这一步用gencode.lncRNA.gtf生成的文件中,cds文件就已经是空的了,后续用metaplots直接报错no orf出不了结果了 上面是lncRNA的gtf生成的,下面是完整gtf生成的
The text was updated successfully, but these errors were encountered:
您好,请问您解决了吗?我遇到了同样的问题。
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prepare_transcripts这一步用gencode.lncRNA.gtf生成的文件中,cds文件就已经是空的了,后续用metaplots直接报错no orf出不了结果了 上面是lncRNA的gtf生成的,下面是完整gtf生成的 您好,请问您解决了吗?我遇到了同样的问题。
得用全转录本,lncRNA没有CDS注释的
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prepare_transcripts这一步用gencode.lncRNA.gtf生成的文件中,cds文件就已经是空的了,后续用metaplots直接报错no orf出不了结果了
![屏幕截图 2024-02-07 152137](https://private-user-images.githubusercontent.com/31724229/302893221-896f3dc2-4b4f-4601-af3c-cb7b04036acc.png?jwt=eyJhbGciOiJIUzI1NiIsInR5cCI6IkpXVCJ9.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.6_-sk5lpvCMxVXj1OgnW4J5BJCeH8omV46RTz0YH26o)
上面是lncRNA的gtf生成的,下面是完整gtf生成的
The text was updated successfully, but these errors were encountered: