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snakemake-pipeline-general

@Jianyu Yang, Pennsylvania State University

General rules could be used for specific purpose

The following files could be expected from this general pipeline:

  • output/mapped/{sample}-{rep}.merge.bam
  • output/mapped/{sample}-{rep}-{unit}.flag.bam
  • output/qc/multiqc/multiqc.html
  • output/qc/bamPEFragmentSize/{sample}-{rep}.hist.png
  • output/coverage/{sample}-{rep}.bgToBw.bw
  • output/coverage/{sample}-{rep}.bamCov.bw
  • output/coverage/{sample}-{rep}.bamCompare.bw

Workflows supported by this general pipeline:

Name Description
chip-seq-standard-pipeline For ChIP-seq, MNase-seq analysis
atac-seq-standard-pipeline For ATAC-seq analysis
dna-methylation-pipeline For BS-seq, TAB-seq, methylC-seq (Bismark seems not working during some steps due to the environment issue)

Change log:

  • Feb 25, 2022: Job grouping for cluster execution (Reverted due to cpu management performance issue)
  • Dec 21, 2021: Signal coverage profile around TSS integrated
  • Nov 23, 2021: Snakemake report feature integrated
  • Nov 20, 2021: All snakemake pipelines have been integrated with cookiecutter, to motivates easily deployment of pipeline

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General rules could be used for specific purpose

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