Xtools package

The ”Xtools” package contains some handy tools for my daily use. Some of them are:

• calculating LT50s (half leathal temperature) with EL (electrolyte leakage) data
• plotting LT50 curves
• processing qPCR data with “HTqPCR” and “ddCt” packages from BioConductor
• set significant labels for multi comparisons: if the labels of two samples have any overlapped letter, the difference between the samples is not significant.

You can use these tools freely if you find them helpful.

---

“Xtools” 是一个 R 软件包，主要为个人数据分析使用。一些有用的工具包括（不限于）：

• 使用 EL（电导率渗漏）数据计算 LT50（半致死温度）
• 绘制 LT50 曲线
• 使用 BioC 的 HTqPCR 和 ddCt 软件包处理 qPCR 数据
• 设置多样本数据比较的显著性标记：如果两样本的有任一相同字母标记，则此对样本无显著差异

如果这些工具有用，您可以自由使用它们。

---

Installation

The package can be install with devtools from github by execute the following commands in R:

## install.packages('devtools')
require('devtools')
install_github('zgzhao/Xtools')

---

About LT50 (half lethal temperature) calculation

We use electrolyte leakage data to calculate LT50. The functions are integrated in a shiny app:

require('Xtools')
LT50s.shiny.app()

It should look like this:

About qPCR data analysis

Data (Ct values) analysis are carried out with HTqPCR and ddCt R packages. They are integrated into a single shiny app in the Xtools package. To run the app, please run the following codes in R console or RStudio:

require('Xtools')
qPCR.shiny.app()

The shiny web app will run locally. It should look like this:

Other functions and tools

Please refer to the package man page after installation.

---