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UW NAPE Imaging Analysis/Preprocessing

Welcome to the UW NAPE (Neurobiology of Addiction, Pain, and Emotion) Center's calcium imaging analysis repository. You will find code for preprocessing calcium imaging (primarily 2-photon, GRIN lens-based deep brain imaging) data.

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Image created by Morgan Alexander

Getting Started

These instructions will get you a copy of the project up and running on your local machine for development and testing purposes.

Prerequisites

Python 2.7
h5py==2.10.0
future==0.14.3
numpy==1.16.5
Pillow==6.2.0
scikit-image==0.14.5
scikit-learn==0.20.4
scipy==1.2.2
Shapely==1.6.4.post2

Installation and Usage

Follow the links below for step by step tutorials on how to install anaconda (python), the environment with the prerequisites, and run the code:

The main jupyter notebook script for the preprocessing pipeline (main_parallel.ipynb) resides in the napeca folder. The main Bruker/tiff prepreprocessing script is in the napeca/prepreprocess folder.

Built With

  • SIMA - Adapted motion correction algorithm; Kaifosh et al., 2014

Authors

If the code and resources outlined here are helpful to your project, we encourage you to cite/acknowledge the UW NAPE Imaging Core. Thank you!

License

This project is licensed under the MIT License - see the LICENSE.md file for details