Thesis R scripts

R scripts used to analyze qPCR data of defence response genes in chickpea:

• qPCR_statistical_analysis.R - This file is used to read raw qPC data and perform ddCq calculation between experimantal groups and test for significance using ANOVA.
• RGA_qPCR_heatmap.R - This file is used to create a heatmap of ddCq values between samples.
• qPCR_data_processing.R - This file is used to import raw qPCR data from each plate, combine them to a single spreadsheet, convert it to a format suitable for LinRegPCR and Factor-qPCR and produce gene expression plots.