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The NEAT Project v3.0

Welcome to the NEAT project, the NExt-generation sequencing Analysis Toolkit, version 3.0. NEAT has now been updated to Python 3, and is moving toward compliance with PEP8 standards. There is still lots of work to be done. See the ChangeLog for notes.

NOTE: Future development on this project will take place in the NCSA project space. The new repo can be found here: Please direct future issues and feature requests to that repository.

Stay tuned over the coming weeks for exciting updates to NEAT, and learn how to contribute yourself. If you'd like to use some of our code, no problem! Just review the license, first.

NEAT's is the main program, and it is a fine-grained read simulator. It simulates real-looking data using models learned from specific datasets. There are several supporting utilities for generating models used for simulation and for comparing the outputs of alignment and variant callers to the golden BAM and golden VCF produced by NEAT.

This is an in-progress v3.0 of the software. Version 2.1 was coded in Python 2, and is available under releases. For an older stable release please see: genReads1

To cite this work, please use:

Stephens, Zachary D., Matthew E. Hudson, Liudmila S. Mainzer, Morgan Taschuk, Matthew R. Weber, and Ravishankar K. Iyer. "Simulating next-generation sequencing datasets from empirical mutation and sequencing models." PloS one 11, no. 11 (2016): e0167047.

Table of Contents


  • Python >= 3.6
  • biopython >= 1.78
  • matplotlib >= 3.3.4 (optional, for plotting utilities)
  • matplotlib_venn >= 0.11.6 (optional, for plotting utilities)
  • pandas >= 1.2.1
  • numpy >= 1.19.5
  • pysam >=


Here's the simplest invocation of genReads using default parameters. This command produces a single ended fastq file with reads of length 101, ploidy 2, coverage 10X, using the default sequencing substitution, GC% bias, and mutation rate models.

python -r ref.fa -R 101 -o simulated_data

The most commonly added options are --pe, --bam, --vcf, and -c.

Option Description
-h, --help Displays usage information
-r Reference sequence file in fasta format. A reference index (.fai) will be created if one is not found in the directory of the reference as [reference filename].fai. Required. The index can be created using samtools faidx.
-R Read length. Required.
-o Output prefix. Use this option to specify where and what to call output files. Required
-c Average coverage across the entire dataset. Default: 10
-e Sequencing error model pickle file
-E Average sequencing error rate. The sequencing error rate model is rescaled to make this the average value.
-p Sample Ploidy, default 2
-tr Bed file containing targeted regions; default coverage for targeted regions is 98% of -c option; default coverage outside targeted regions is 2% of -c option
-dr Bed file with sample regions to discard.
-to off-target coverage scalar [0.02]
-m mutation model pickle file
-M Average mutation rate. The mutation rate model is rescaled to make this the average value. Must be between 0 and 0.3. These random mutations are inserted in addition to the once specified in the -v option.
-Mb Bed file containing positional mutation rates
-N Below this quality score, base-call's will be replaced with N's
-v Input VCF file. Variants from this VCF will be inserted into the simulated sequence with 100% certainty.
--pe Paired-end fragment length mean and standard deviation. To produce paired end data, one of --pe or --pe-model must be specified.
--pe-model Empirical fragment length distribution. Can be generated using To produce paired end data, one of --pe or --pe-model must be specified.
--gc-model Empirical GC coverage bias distribution. Can be generated using
--bam Output golden BAM file
--vcf Output golden VCF file
--fa Output FASTA instead of FASTQ
--rng rng seed value; identical RNG value should produce identical runs of the program, so things like read locations, variant positions, error positions, etc, should all be the same.
--gz Gzip output FQ and VCF
--no-fastq Bypass generation of FASTQ read files
--discard-offtarget Discard reads outside of targeted regions
--rescale-qual Rescale Quality scores to match -E input
-d Turn on debugging mode (useful for development)


Diagram describing the way that genReads simulates datasets

NEAT produces simulated sequencing datasets. It creates FASTQ files with reads sampled from a provided reference genome, using sequencing error rates and mutation rates learned from real sequencing data. The strength of NEAT lies in the ability for the user to customize many sequencing parameters, produce 'golden,' true positive datasets. We are working on expanding the functionality even further to model more species, generate larger variants, model tumor/normal data and more!


  • Simulate single-end and paired-end reads
  • Custom read length
  • Can introduce random mutations and/or mutations from a VCF file
    • Supported mutation types include SNPs, indels (of any length), inversions, translocations, duplications
    • Can emulate multi-ploid heterozygosity for SNPs and small indels
  • Can simulate targeted sequencing via BED input specifying regions to sample from
  • Can accurately simulate large, single-end reads with high indel error rates (PacBio-like) given a model
  • Specify simple fragment length model with mean and standard deviation or an empirically learned fragment distribution using utilities/
  • Simulates quality scores using either the default model or empirically learned quality scores using utilities/
  • Introduces sequencing substitution errors using either the default model or empirically learned from utilities/
  • Accounts for GC% coverage bias using model learned from utilities/
  • Output a VCF file with the 'golden' set of true positive variants. These can be compared to bioinformatics workflow output (includes coverage and allele balance information)
  • Output a BAM file with the 'golden' set of aligned reads. These indicate where each read originated and how it should be aligned with the reference
  • Create paired tumour/normal datasets using characteristics learned from real tumour data
  • Low memory footprint. Constant (proportional to the size of the reference sequence)


The following commands are examples for common types of data to be generated. The simulation uses a reference genome in fasta format to generate reads of 126 bases with default 10X coverage. Outputs paired fastq files, a BAM file and a VCF file. The random variants inserted into the sequence will be present in the VCF and all of the reads will show their proper alignment in the BAM. Unless specified, the simulator will also insert some "sequencing error" -- random variants in some reads that represents false positive results from sequencing.

Whole genome simulation

Simulate whole genome dataset with random variants inserted according to the default model.

python                  \
        -r hg19.fa                  \
        -R 126                      \
        -o /home/me/simulated_reads \
        --bam                       \
        --vcf                       \
        --pe 300 30

Targeted region simulation

Simulate a targeted region of a genome, e.g. exome, with -tr.

python                  \
        -r hg19.fa                  \
        -R 126                      \
        -o /home/me/simulated_reads \
        --bam                       \
        --vcf                       \
        --pe 300 30                 \
        -tr hg19_exome.bed

Insert specific variants

Simulate a whole genome dataset with only the variants in the provided VCF file using -v and -M.

python                  \
        -r hg19.fa                  \
        -R 126                      \
        -o /home/me/simulated_reads \
        --bam                       \
        --vcf                       \
        --pe 300 30                 \
        -v NA12878.vcf              \
        -M 0

Single end reads

Simulate single-end reads by omitting the --pe option.

python                  \
        -r hg19.fa                  \
        -R 126                      \
        -o /home/me/simulated_reads \
        --bam                       \

Large single end reads

Simulate PacBio-like reads by providing an error model.

python                         \
	-r hg19.fa                         \
	-R 5000                            \
	-e models/errorModel_pacbio_toy.p  \
	-E 0.10                            \
	-o /home/me/simulated_reads        


Several scripts are distributed with gen_reads that are used to generate the models used for simulation.

Computes GC% coverage bias distribution from sample (bedrolls genomecov) data. Takes .genomecov files produced by BEDtools genomeCov (with -d option).

bedtools genomecov
        -d                          \
        -ibam normal.bam            \
        -g reference.fa
python                 \
        -r reference.fa             \
        -i genomecovfile            \
        -w [sliding window length]  \
        -o /path/to/model.p

Computes empirical fragment length distribution from sample data. Takes SAM file via stdin:

./samtools view toy.bam | python

and creates fraglen.p model in working directory.

Takes references genome and TSV file to generate mutation models:

python               \
        -r hg19.fa                  \
        -m inputVariants.tsv        \
        -o /home/me/models.p

Trinucleotides are identified in the reference genome and the variant file. Frequencies of each trinucleotide transition are calculated and output as a pickle (.p) file.

Option Description
-r Reference file for organism in FASTA format. Required
-m Mutation file for organism in VCF format. Required
-o Path to output file and prefix. Required.
-b BED file of regions to include
--save-trinuc Save trinucleotide counts for reference
--human-sample Use to skip unnumbered scaffolds in human references
--skip-common Do not save common snps or high mutation areas

Generates sequence error model for -e option. This script needs revision, to improve the quality-score model eventually, and to include code to learn sequencing errors from pileup data.

python                            \
        -i input_read1.fq (.gz) / input_read1.sam     \
        -o output.p                                   \
        -i2 input_read2.fq (.gz) / input_read2.sam    \
        -p input_alignment.pileup                     \
        -q quality score offset [33]                  \
        -Q maximum quality score [41]                 \
        -n maximum number of reads to process [all]   \
        -s number of simulation iterations [1000000]  \
        --plot perform some optional plotting

Performs plotting and comparison of mutation models generated from

python                                        \
        -i model1.p [model2.p] [model3.p]...                  \
        -l legend_label1 [legend_label2] [legend_label3]...   \
        -o path/to/pdf_plot_prefix

Tool for comparing VCF files. This program needs updates, as it is slow and somewhat touchy to use at the moment.

        -r <ref.fa>        * Reference Fasta                           \
        -g <golden.vcf>    * Golden VCF                                \
        -w <workflow.vcf>  * Workflow VCF                              \
        -o <prefix>        * Output Prefix                             \
        -m <track.bed>     Mappability Track                           \
        -M <int>           Maptrack Min Len                            \
        -t <regions.bed>   Targetted Regions                           \
        -T <int>           Min Region Len                              \
        -c <int>           Coverage Filter Threshold [15]              \
        -a <float>         Allele Freq Filter Threshold [0.3]          \
        --vcf-out          Output Match/FN/FP variants [False]         \
        --no-plot          No plotting [False]                         \
        --incl-homs        Include homozygous ref calls [False]        \
        --incl-fail        Include calls that failed filters [False]   \
        --fast             No equivalent variant detection [False]

Mappability track examples:

Note on Sensitive Patient Data

ICGC's "Access Controlled Data" documention can be found at To have access to controlled germline data, a DACO must be submitted. Open tier data can be obtained without a DACO, but germline alleles that do not match the reference genome are masked and replaced with the reference allele. Controlled data includes unmasked germline alleles.