New in this release:
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Visualization/GUI improvements:
- Added option to include foci outside nuclei/cells. The distance range can be specified.
- Included option to detect foci on extended depth of focus projection (Sobel, GPU)
- 3D image options are now radiobuttons instead of separate checkboxes
- Downscaled nuclei masks are now smoothed using spline fitting (StarDist only)
- If the 'Alt' key is pressed before a file is loaded, the user can select a ROI to do a preview analysis on.
- Added a cellpose diameter parameter (was set to default (30 pixels) before)
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Output improvements:
- The actually used threshold value (Threshold factor * bias) is now mentioned in the log window and added to the output image metadata.
- Colocalization overlap image is now also 3D and saved as .Gif file to save space
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Bug fixes:
- Fixed a bug causing a crash with 32-bit images when producing the overlay.
The macro Merge_result_files_1.1 (see below) can be used to merge all foci results .tsv files in a folder into a single table and .tsv file.