This project was developed for the realtime analysis of C. elegans morphologic features of length/width/volume/curvature during free moving on agar substrate. Based on the analysis results, the entire worm body could be divided into ~100 segments on both dorsal and ventral side. The overall time cost of a single image frame takes less than 2 ms.
NOTE: If you use any portion of this code in your research, kindly cite: [1] Xianke Dong, Pengfei Song, and Xinyu Liu. "An Automated Microfluidic System for Morphological Measurement and Size-Based Sorting of C. Elegans." IEEE transactions on nanobioscience (2019).
and our following works on C. elegans light-driven microrobot: [2] Xianke Dong, Sina Kheiri, Yangning Lu, Zhaoyi Xu, Mei Zhen, Xinyu Liu, "Toward a living soft microrobot through optogenetic locomotion control of Caenorhabditis elegans." Science Robotics 6, eabe3950 (2021).