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filter-out option no work for SE reads #453

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y9c opened this issue Jan 25, 2021 · 4 comments
Closed

filter-out option no work for SE reads #453

y9c opened this issue Jan 25, 2021 · 4 comments

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@y9c
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y9c commented Jan 25, 2021

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@timedreamer
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Hi, I'm facing this problem also. Any solutions so far?
I got the error UnboundLocalError: local variable 'read1' referenced before assignment

Thanks!
Ji

@IanSudbery
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Hi, I just merged the PR from @yech1990 that should fix this. If you are able to install the latest from master, it should work. Let me know if you need help with that.

@timedreamer
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timedreamer commented Feb 1, 2021

Hey great! If you could help with installing from master, that would be great! I used conda to install before. I can wait for the conda version too, if it doesn't take long. Thanks!

@IanSudbery
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Hi @timedreamer

I don't know how long the conda release will be as @TomSmithCGAT normally rolls these once there are a few changes to bundle up.

To install from master:

  1. Activate your conda environment.
  2. Download/clone the code to any location of your choice with:
    git clone https://github.com/CGATOxford/UMI-tools.git
  3. Enter the UMI-tools directory:
    cd UMI-tools
  4. Install:
    python setup.py install

You can delete the downloaded/cloned directory if you wish at this point.

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