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Merge pull request #110 from liujamin/patch-1
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rob-p committed Mar 31, 2023
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Expand Up @@ -4,7 +4,7 @@ Getting Started
Running the alevin-fry pipeline
-------------------------------

First, we need to generate a RAD file using alevin. The RAD file is created by mapping the sequencing reads against an index of the reference. We recommend using a `splici <https://combine-lab.github.io/alevin-fry-tutorials/2021/improving-txome-specificity/>`_ reference index. The mappings can be generated using either `selective-alignemnt <https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-02151-8>`_ or `pseudoalignemnt <https://www.nature.com/articles/nbt.3519>`_ against the transcriptiome (with the ``--rad`` or ``--sketch`` flags, respectively). **Note**, however, that alevin-fry does not currently support RAD files aligned against a decoy-aware index, so that indices used for RAD file generation should be prepared without decoy sequnece. For a chromium v2 set of read files, the command would look like the following:
First, we need to generate a RAD file using alevin. The RAD file is created by mapping the sequencing reads against an index of the reference. We recommend using a `splici <https://combine-lab.github.io/alevin-fry-tutorials/2021/improving-txome-specificity/>`_ reference index. The mappings can be generated using either `selective-alignment <https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-02151-8>`_ or `pseudoalignment <https://www.nature.com/articles/nbt.3519>`_ against the transcriptome (with the ``--rad`` or ``--sketch`` flags, respectively). **Note**, however, that alevin-fry does not currently support RAD files aligned against a decoy-aware index, so that indices used for RAD file generation should be prepared without decoy sequnece. For a chromium v2 set of read files, the command would look like the following:

.. code:: bash
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