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Update salmon.rst
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rob-p committed Jul 12, 2015
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Expand Up @@ -94,12 +94,20 @@ you run the Salmon indexer:
> ./bin/salmon index -t transcripts.fa -i transcripts_index

Then, you can quantify any set of reads (say, paired-end reads in files
`reads1.fa` and `reads2.fa`) directly against this index using the Salmon
`reads1.fq` and `reads2.fq`) directly against this index using the Salmon
``quant`` command as follows:

::

> ./bin/salmon quant -i transcripts_index -l <LIBTYPE> -1 reads1.fa -2 reads2.fa -o transcripts_quant
> ./bin/salmon quant -i transcripts_index -l <LIBTYPE> -1 reads1.fq -2 reads2.fq -o transcripts_quant

If you are using single-end reads, then you pass them to Salmon with
the ``-r`` flag like:

::

> ./bin/salmon quant -i transcripts_index -l <LIBTYPE> -r reads.fq -o transcripts_quant


You can, of course, pass a number of options to control things such as the
number of threads used or the different cutoffs used for counting reads.
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