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FrancisCrickInstitute/Embryo-micro-injection-chamber

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Embryo-micro-injection-chamber

Acrylic (PMMA) mould for PDMS casting designed and manufactured in colaboration with Aurélien Courtois from the Turner lab at the Crick.

The mould is composed by 4 parts which are laser cut in 3 mm thickness.

The four parts of the mould are attached using M3 screws.

We have used PDMS in 10:1 ratio with the curing agent.

Use a pasteur pipette to pour the PDMS inside the mould.

Cure the PDMS at 65°C for 2h.

After removing the PDMS from the mould we plasma bond it with the coverslip.

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Mould to build a PDMS vessel for embryo micro-manipulation/imaging

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