ENA Metagenome Gene Catalog

Download metagenomic assemblies from ENA and assemble a gene catalog

Workflow

1. Identify all of the host-associated metagenomes with the ENA API
2. Download each metagenome assembly and extract the CDS (gene sequences)
3. Aggregate all gene sequences
4. Cluster genes at a few different levels of amino acid identity
5. Return the non-redundant gene catalogs

Output

For each level of amino acid identity (e.g. 100%, 90%, 80%):

• FASTA of protein sequences
• TSV linking each input gene to the final cluster