Amazon Web Services Tutorials (For Scientists)!

Go back to GBI AWS Wiki

Just like the Command Line Tutorial, there are 2 modules for the Amazon Web Services Tutorial as well. However, instead of the second directly building off of the first, these two modules will be quite a bit different.

After completing the CLI tutorial, you should have a good foundation with navigating around and manipulating files and directories within the command line. The first module in this tutorial will shift slightly and focus on the fundamentals of AWS. What is it? Why will we use it? How do we use it for analysis? The second module in this tutorial will then wrap up everything we have done by using our new CLI skills and AWS knowledge to go take you through an entire genome assembly pipeline from just having your sequencing data to having the resulting assembly with information and statistics describing it.

Module 1 - Fundamentals of Amazon Web Services:

• Section 1: Intro to Amazon Web Services, Identity & Access Management (IAM), and Billing
• Section 2: Elastic Cloud Computing (EC2) and Elastic Block Store (EBS)
• Section 3: Simple Storage Service (S3) and the AWS CLI
• Section 4: Amazon Machine Images (AMI), the GBI AMI, and the GBI AWS Github as a Resource

Module 2 - Genome Analysis and Assembly Walkthrough:

• Section 5: Setting up EC2 instance and uploading data
• Section 6: Pre-assembly softwares
• Section 7: Genome assembly with ABySS and SOAPdenovo2
• Section 8: Checking our assembly quality, understanding our results, downloading data, and shutting down our instance

Conclusion:

• Section 9: Wrapping it all up (all review questions)