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An open-source Optical projection tomography platform for effective sampling of zebrafish embyros. Codes are based on MATLAB software with an GUI and a generalized automated workflow can provide accurate and high-quality 3D reconstructions.

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zOPT

This project contains an open-source implementation of an OPT platform. The 3D-printable components are provided to built your customized sample stage. We offer our open source MATLAB software with an GUI for OPT imaging. We developed a generalized automated workflow including a two-step registration approach for correcting the center of rotation and provide accurate and high-quality 3D reconstruction.

Here we provide the source codes and a user guide to built up the zOPT system. For sample data from the system, please follow this link, https://sourceforge.net/projects/zopt-tomography-reconstruction. We saved a bright field OPT tomography data recorded by our zOPT setup and it can be used to verify the algorithms for zOPT tomography reconstruction.

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An open-source Optical projection tomography platform for effective sampling of zebrafish embyros. Codes are based on MATLAB software with an GUI and a generalized automated workflow can provide accurate and high-quality 3D reconstructions.

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