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LAND is a software package to quantitatively analyze single molecule localization microscopy (SMLM) data. The package includes algorithms to analyze the clustering of molecules and their spatial distribution. In addition, it contains algorithms specifically designed to analyze the nuclear nanostructure. LAND is designed to evaluate large sample sizes and data with high emitter densities. It is intended to support researchers as a tool

• for a quick initial analysis of the experiments
• to optimize the analysis parameters
• for routine applications with an optimized set of parameters
• to proceed after inital analysis with additional specialized algorithms

Due to the large number of different kind of SMLM experiments, custom-tailored pre- and post-processing routines may be necessary. Examples for different applications are shown in ??. For the experienced user, who intends to extend the capabilities of LAND with custom routines, we provide an interface to access its core functionality (??).