-
Notifications
You must be signed in to change notification settings - Fork 0
Commit
This commit does not belong to any branch on this repository, and may belong to a fork outside of the repository.
- Loading branch information
Kohze
committed
Mar 21, 2017
1 parent
d3da279
commit 8eb0696
Showing
11 changed files
with
275 additions
and
579 deletions.
There are no files selected for viewing
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
| Original file line number | Diff line number | Diff line change |
|---|---|---|
| @@ -1,5 +1,11 @@ | ||
| # Frapbot | ||
| #www.frapbot.com | ||
|
|
||
| Demo dataset is available at | ||
| download.frapbot.com | ||
| ---- | ||
|
|
||
| Frapbot is a R & Shiny based, open source analysis toolbox for FRAP experiments. | ||
|
|
||
| www.frapbot.com | ||
|
|
||
|
|
||
|
|
||
|  |
File renamed without changes.
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
| Original file line number | Diff line number | Diff line change |
|---|---|---|
| @@ -0,0 +1,251 @@ | ||
| <!DOCTYPE html> | ||
| <html dir="ltr" lang="en-US"> | ||
| <head> | ||
|
|
||
| <meta http-equiv="content-type" content="text/html; charset=utf-8" /> | ||
| <meta name="Robin Kohze" content="FrapBot" /> | ||
|
|
||
| <!-- Stylesheets | ||
| ============================================= --> | ||
| <link href="http://fonts.googleapis.com/css?family=Lato:300,400,400italic,600,700|Raleway:300,400,500,600,700|Crete+Round:400italic" rel="stylesheet" type="text/css" /> | ||
| <link rel="stylesheet" href="css/bootstrap.css" type="text/css" /> | ||
| <link rel="stylesheet" href="style.css" type="text/css" /> | ||
| <link rel="stylesheet" href="css/dark.css" type="text/css" /> | ||
| <link rel="stylesheet" href="css/font-icons.css" type="text/css" /> | ||
| <link rel="stylesheet" href="css/animate.css" type="text/css" /> | ||
| <link rel="stylesheet" href="css/magnific-popup.css" type="text/css" /> | ||
| <link rel="stylesheet" href="css/imports/header.css" type="text/css" /> | ||
| <link rel="stylesheet" href="css/image-map-pro.min.css"> | ||
|
|
||
|
|
||
| <link rel="stylesheet" href="css/responsive.css" type="text/css" /> | ||
| <meta name="viewport" content="width=device-width, initial-scale=1" /> | ||
| <!--[if lt IE 9]> | ||
| <script src="http://css3-mediaqueries-js.googlecode.com/svn/trunk/css3-mediaqueries.js"></script> | ||
| <![endif]--> | ||
|
|
||
| <!-- External JavaScripts | ||
| ============================================= --> | ||
| <script type="text/javascript" src="js/jquery.js"></script> | ||
| <script type="text/javascript" src="js/plugins.js"></script> | ||
|
|
||
|
|
||
| <!-- Document Title | ||
| ============================================= --> | ||
| <title>FAQs with Side-by-Side Questions | Canvas</title> | ||
|
|
||
|
|
||
|
|
||
| </head> | ||
|
|
||
| <body class="stretched"> | ||
|
|
||
| <!-- Document Wrapper | ||
| ============================================= --> | ||
| <div id="wrapper" class="clearfix"> | ||
|
|
||
| <!-- Header | ||
| ============================================= --> | ||
| <header id="header" class="full-header static-sticky"> | ||
|
|
||
|
|
||
|
|
||
| <div id="header-wrap"> | ||
|
|
||
| <div class="container clearfix"> | ||
|
|
||
| <div id="primary-menu-trigger"><i class="icon-reorder"></i></div> | ||
|
|
||
| <!-- Logo | ||
| ============================================= --> | ||
| <div id="logo"> | ||
| <a href="index.html" class="standard-logo" data-dark-logo="images/logo-dark.png"><img src="images/logo.png" alt="Canvas Logo"></a> | ||
| <a href="index.html" class="retina-logo" data-dark-logo="images/logo-dark@2x.png"><img src="images/logo@2x.png" alt="Canvas Logo"></a> | ||
| </div><!-- #logo end --> | ||
|
|
||
| <!-- Primary Navigation | ||
| ============================================= --> | ||
| <nav id="primary-menu"> | ||
|
|
||
| <ul> | ||
| <li><a href="index.html"><div>FrapBot</div></a> | ||
|
|
||
| </li> | ||
| <li><a href="features.html"><div>Features</div></a> | ||
|
|
||
| </li> | ||
| <li><a href="paper.html"><div>The Paper</div></a> | ||
|
|
||
| </li> | ||
| <li><a href="faq.html"><div>FAQ</div></a> | ||
|
|
||
| </li> | ||
| <li><a href="update-log.html"><div>Update Log</div></a> | ||
|
|
||
| </li> | ||
|
|
||
|
|
||
| </ul> | ||
|
|
||
| <!-- Top Cart | ||
| ============================================= --> | ||
|
|
||
|
|
||
| <!-- Top Search | ||
| ============================================= --> | ||
|
|
||
|
|
||
| </nav><!-- #primary-menu end --> | ||
|
|
||
| </div> | ||
|
|
||
| </div> | ||
|
|
||
| </header><!-- #header end --> | ||
|
|
||
| <!-- Page Title | ||
| ============================================= --> | ||
|
|
||
|
|
||
| <!-- Content | ||
| ============================================= --> | ||
| <section id="content"> | ||
|
|
||
|
|
||
|
|
||
| <div style="background-color: #F9f9f9; padding: 40px;"> | ||
| <div class="container clearfix" style=" padding: 40px;"> | ||
|
|
||
| <div class="col_two_fifth nobottommargin"> | ||
|
|
||
| <div class="masonry-thumbs col-2 clearfix" data-big="1" style="padding-top: 60px;"> | ||
| <a href="images/example/Dieteren.jpg" data-lightbox="image"> | ||
| <img src="images/example/Dieteren.jpg" alt="Single Image"> | ||
| <div class="overlay"><div class="overlay-wrap"><i class="icon-line-plus"></i></div></div> | ||
| </a> | ||
| </div> | ||
| </div> | ||
|
|
||
| <div class="col_three_fifth nobottommargin col_last"> | ||
|
|
||
| <h3>Dieteren et al. </h3> | ||
|
|
||
| <div class="masonry-thumbs col-5 clearfix" data-big="3" data-lightbox="gallery"> | ||
| <p> We benchmarked Frapbot using a previously published FRAP dataset obtained with inducible human embryonal | ||
| kidney (HEK293) cells that expressed a monomeric green fluorescent protein (AcGFP1) in the mitochondrial | ||
| matrix (6). In the original study, the average trace of multiple FRAP measurements (N=187) was manually fitted | ||
| with a single component exponential model by Origin Pro software, delivering a τmono value of 0.600 s, | ||
| corresponding to a t1/2 of 0.416 s. Individual analysis of the identical FRAP traces by Frapbot (Fig. 3, A) and | ||
| subsequent averaging of the obtained t1/2 values, delivered a slightly higher mean halftime (t1/2) of 0.463 s (Fig. | ||
| 3, B). This difference might be explained by the larger noise of the individual traces compared to the averaged | ||
| FRAP curve, that influences the quality of the fitting. Frapbot analysis suggests that the t1/2 values of the dataset | ||
| were not Gaussian distributed. If this is the case, we recommend the use of the median instead of the average for | ||
| the analysis of similar future FRAP data which provides more robustness against outliers. Consequently, the | ||
| calculation of the median was implemented in Frapbot (Fig. 2, yellow box, plot 11). </p> | ||
| </div> | ||
| <a href="examples.html" class="button button-border button-rounded button-dark noleftmargin">Download Dataset</a> | ||
|
|
||
|
|
||
| </div> | ||
|
|
||
| </div> | ||
| </div> | ||
|
|
||
| <div style="background-color: #FFFFFF; padding: 40px;"> | ||
| <div class="container clearfix" style=" padding: 40px;"> | ||
|
|
||
| <div class="col_two_fifth nobottommargin"> | ||
|
|
||
| <div class="masonry-thumbs col-2 clearfix" data-big="1" style="padding-top: 60px;"> | ||
| <a href="images/example/Dieteren.jpg" data-lightbox="image"> | ||
| <img src="images/example/Dieteren.jpg" alt="Single Image"> | ||
| <div class="overlay"><div class="overlay-wrap"><i class="icon-line-plus"></i></div></div> | ||
| </a> | ||
| </div> | ||
| </div> | ||
|
|
||
| <div class="col_three_fifth nobottommargin col_last"> | ||
|
|
||
| <h3>Dieteren et al. </h3> | ||
|
|
||
| <div class="masonry-thumbs col-5 clearfix" data-big="3" data-lightbox="gallery"> | ||
| <p> We benchmarked Frapbot using a previously published FRAP dataset obtained with inducible human embryonal | ||
| kidney (HEK293) cells that expressed a monomeric green fluorescent protein (AcGFP1) in the mitochondrial | ||
| matrix (6). In the original study, the average trace of multiple FRAP measurements (N=187) was manually fitted | ||
| with a single component exponential model by Origin Pro software, delivering a τmono value of 0.600 s, | ||
| corresponding to a t1/2 of 0.416 s. Individual analysis of the identical FRAP traces by Frapbot (Fig. 3, A) and | ||
| subsequent averaging of the obtained t1/2 values, delivered a slightly higher mean halftime (t1/2) of 0.463 s (Fig. | ||
| 3, B). This difference might be explained by the larger noise of the individual traces compared to the averaged | ||
| FRAP curve, that influences the quality of the fitting. Frapbot analysis suggests that the t1/2 values of the dataset | ||
| were not Gaussian distributed. If this is the case, we recommend the use of the median instead of the average for | ||
| the analysis of similar future FRAP data which provides more robustness against outliers. Consequently, the | ||
| calculation of the median was implemented in Frapbot (Fig. 2, yellow box, plot 11). </p> | ||
| </div> | ||
| <a href="examples.html" class="button button-border button-rounded button-dark noleftmargin">Download Dataset</a> | ||
|
|
||
|
|
||
| </div> | ||
|
|
||
| </div> | ||
| </div> | ||
| <div style="background-color: #F9f9f9; padding: 40px;"> | ||
| <div class="container clearfix" style="padding: 40px;"> | ||
|
|
||
| <div class="col_two_fifth nobottommargin"> | ||
|
|
||
| <div class="masonry-thumbs col-2 clearfix" data-big="1" style="padding-top: 60px;"> | ||
| <a href="images/example/Dieteren.jpg" data-lightbox="image"> | ||
| <img src="images/example/Dieteren.jpg" alt="Single Image"> | ||
| <div class="overlay"><div class="overlay-wrap"><i class="icon-line-plus"></i></div></div> | ||
| </a> | ||
| </div> | ||
| </div> | ||
|
|
||
| <div class="col_three_fifth nobottommargin col_last"> | ||
|
|
||
| <h3>Dieteren et al. </h3> | ||
|
|
||
| <div class="masonry-thumbs col-5 clearfix" data-big="3" data-lightbox="gallery"> | ||
| <p> We benchmarked Frapbot using a previously published FRAP dataset obtained with inducible human embryonal | ||
| kidney (HEK293) cells that expressed a monomeric green fluorescent protein (AcGFP1) in the mitochondrial | ||
| matrix (6). In the original study, the average trace of multiple FRAP measurements (N=187) was manually fitted | ||
| with a single component exponential model by Origin Pro software, delivering a τmono value of 0.600 s, | ||
| corresponding to a t1/2 of 0.416 s. Individual analysis of the identical FRAP traces by Frapbot (Fig. 3, A) and | ||
| subsequent averaging of the obtained t1/2 values, delivered a slightly higher mean halftime (t1/2) of 0.463 s (Fig. | ||
| 3, B). This difference might be explained by the larger noise of the individual traces compared to the averaged | ||
| FRAP curve, that influences the quality of the fitting. Frapbot analysis suggests that the t1/2 values of the dataset | ||
| were not Gaussian distributed. If this is the case, we recommend the use of the median instead of the average for | ||
| the analysis of similar future FRAP data which provides more robustness against outliers. Consequently, the | ||
| calculation of the median was implemented in Frapbot (Fig. 2, yellow box, plot 11). </p> | ||
| </div> | ||
| <a href="examples.html" class="button button-border button-rounded button-dark noleftmargin">Download Dataset</a> | ||
|
|
||
|
|
||
| </div> | ||
|
|
||
| </div> | ||
| </div> | ||
|
|
||
| </div> | ||
|
|
||
| </section><!-- #content end --> | ||
|
|
||
|
|
||
| <!-- Footer | ||
| ============================================= --> | ||
|
|
||
|
|
||
| <!-- Copyrights | ||
| ============================================= --> | ||
|
|
||
|
|
||
| <!-- Go To Top | ||
| ============================================= --> | ||
| <div id="gotoTop" class="icon-angle-up"></div> | ||
|
|
||
| <!-- Footer Scripts | ||
| ============================================= --> | ||
| <script type="text/javascript" src="js/functions.js"></script> | ||
|
|
||
| </body> | ||
| </html> |
Oops, something went wrong.