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Before #8 and likely also before #7, we'll want to add a few things to our sce object. One of them could be a (or more than one) discrete variable https://github.com/LieberInstitute/spatialLIBD/blob/master/R/run_app.R#L58 that tells us what were spots that scran's quality control checks (designed for scRNA-seq data, not Visium data) are of low quality and should be discarded. That is, running code like https://github.com/LieberInstitute/HumanPilot/blob/master/Analysis/sce_scran.R#L32-L33 then taking the outputs (which are logical vectors) and converting them to discrete variables (so factor or character).
sce
scran
logical
factor
character
Let's try this with the 3 columns in https://github.com/LieberInstitute/HumanPilot/blob/master/Analysis/sce_scran.R#L35. So something like:
sce$scran_discard <- factor(edit_me, levels = c("TRUE", "FALSE")) sce$scran_low_lib_size <- ... sce$low_n_features <- ...
sce$scran_discard <- factor(edit_me, levels = c("TRUE", "FALSE"))
sce$scran_low_lib_size <- ...
sce$low_n_features <- ...
This might be more relevant for the LC data than the DLPFC data. But it'll be good to check.
The text was updated successfully, but these errors were encountered:
Fix a serious issue where marker selection had not actually been grou…
791c9dc
…ped by cell type; merge all excitatory and all inhibitory cell types into a single category each; select ~200 unique markers for now. Closes #9.
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Before #8 and likely also before #7, we'll want to add a few things to our
sce
object. One of them could be a (or more than one) discrete variable https://github.com/LieberInstitute/spatialLIBD/blob/master/R/run_app.R#L58 that tells us what were spots thatscran
's quality control checks (designed for scRNA-seq data, not Visium data) are of low quality and should be discarded. That is, running code like https://github.com/LieberInstitute/HumanPilot/blob/master/Analysis/sce_scran.R#L32-L33 then taking the outputs (which arelogical
vectors) and converting them to discrete variables (sofactor
orcharacter
).Let's try this with the 3 columns in https://github.com/LieberInstitute/HumanPilot/blob/master/Analysis/sce_scran.R#L35. So something like:
sce$scran_discard <- factor(edit_me, levels = c("TRUE", "FALSE"))
sce$scran_low_lib_size <- ...
sce$low_n_features <- ...
This might be more relevant for the LC data than the DLPFC data. But it'll be good to check.
The text was updated successfully, but these errors were encountered: