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AMICO

Macros for Image Cytometry Analysis by Fluorescence Microscopy in ImageJ.

The site is under construction. For more information take a look at the references below or write to mario.faretta@ieo.it The macros were tested on ImageJ 1.53n, JAVA 1.8.0_172 (64-bit) on Windows 8.1.

References:

1.Furia L, Pelicci PG, Faretta M. A computational platform for robotized fluorescence microscopy (I): high-content image-based cell-cycle analysis. Cytometry A. 2013 Apr;83(4):333-43. doi: 10.1002/cyto.a.22266. Epub 2013 Mar 5. PMID: 23463605.

2.Furia L, Pelicci PG, Faretta M. A computational platform for robotized fluorescence microscopy (II): DNA damage, replication, checkpoint activation, and cell cycle progression by high-content high-resolution multiparameter image-cytometry. Cytometry A. 2013 Apr;83(4):344-55. doi: 10.1002/cyto.a.22265. Epub 2013 Mar 5. PMID: 23463591.

3.Furia L, Pelicci P, Faretta M. Confocal microscopy for high-resolution and high-content analysis of the cell cycle. Curr Protoc Cytom. 2014 Oct 1;70:7.42.1-14. doi: 10.1002/0471142956.cy0742s70. PMID: 25271962.

4.Furia L, Pelicci P, Faretta M. Confocal microscopy for high-resolution and high-content analysis of the cell cycle. Curr Protoc Cytom. 2014 Oct 1;70:7.42.1-14. doi: 10.1002/0471142956.cy0742s70. PMID: 25271962.

AMICO_Union.txt

Macro to Browse through Acquired Images, Set for Segmentation and Analysis of Particles, Set of intracellular subcompartment (e.g. foci) recognition. Results are stored in a tab-txt file. The macro works on .nd2, multichannel OME.tiff files or on separated channels tiffs for a single position.

AMICO_Plotting.txt

Macro to analyze the results of AMICO_Union Image Analysis producing Dot Plots and Histograms. It is possible to define Regions of Interest and combine them into logical gates as normally done in flow-cytometry.

MacroRegTwoImages_220722.txt

Macro to register two images and multichannel stacks based on the TurboReg ImageJ Plugin

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