Pan-cancer functional annotation of miRNAs
This repository provides all codes used to analyze CRISPR-Cas9 screens using the lentiG-miR (CP1608) library in 47 human cancer cell lines.
Code includes low-level QC, correction for gene-independent cell responses to CRISPR-Cas9 targeting (CRISPRcleanR), functional validation of screen performance, and sgRNA-level and/or gene-level estimation of gene essentiality using BAGEL2 and MAGeCK-RRA. Furthermore, miRNAs with a significant effect in the majority of or all cancer cell lines investigated were determined using the 90th percentile method (common essential miRNA).