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Filament_Tools

Volker edited this page Jan 22, 2021 · 1 revision

Calculate the area-fraction of the image filled with (septin) filaments.

Image%2059_Out-3.png results.png

Getting started

To install the tool save the file filament_tools.ijm into the folder macros/toolsets of your FIJI installation.

Select the "filament tools" toolset from the >> button of the ImageJ launcher.

toolset.png

  • press the a-button to analyze the active image
  • press the b-button to analyze the images in a folder in batch-mode.

Options

Right-click the a-button to open the options dialog.

options1.png

  • filament channel - the number of the channel containing the filament image.
  • min. area - the minimal area an object must have to be considered a filament.
  • max. circularity - allows to remove objects too round to be filaments
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