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Merge pull request #217 from ErikDanielsson/fastqc-keyerror
File to reproduce MultiQC/MultiQC#1533
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| Original file line number | Diff line number | Diff line change |
|---|---|---|
| @@ -0,0 +1,304 @@ | ||
| ##FastQC 0.11.5 | ||
| >>Basic Statistics pass | ||
| #Measure Value | ||
| Filename F1-1A_S1_R1_001.fastq.gz | ||
| File type Conventional base calls | ||
| Encoding Sanger / Illumina 1.9 | ||
| Total Sequences 18529595 | ||
| Sequences flagged as poor quality 0 | ||
| Sequence length 35-151 | ||
| %GC 49 | ||
| >>END_MODULE | ||
| >>Per base sequence quality pass | ||
| #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile | ||
| 1 36.22621357887207 37.0 37.0 37.0 37.0 37.0 | ||
| 2 35.964096355047154 37.0 37.0 37.0 37.0 37.0 | ||
| 3 36.08126837094928 37.0 37.0 37.0 37.0 37.0 | ||
| 4 36.15553993489874 37.0 37.0 37.0 37.0 37.0 | ||
| 5 36.15440461596705 37.0 37.0 37.0 37.0 37.0 | ||
| 6 36.13112143033887 37.0 37.0 37.0 37.0 37.0 | ||
| 7 36.05261286066965 37.0 37.0 37.0 37.0 37.0 | ||
| 8 36.14941886209601 37.0 37.0 37.0 37.0 37.0 | ||
| 9 36.14225972019356 37.0 37.0 37.0 37.0 37.0 | ||
| 10-14 36.17964399113957 37.0 37.0 37.0 37.0 37.0 | ||
| 15-19 36.182579694807146 37.0 37.0 37.0 37.0 37.0 | ||
| 20-24 36.15952688658333 37.0 37.0 37.0 37.0 37.0 | ||
| 25-29 36.0721008850976 37.0 37.0 37.0 37.0 37.0 | ||
| 30-34 36.038086488128855 37.0 37.0 37.0 37.0 37.0 | ||
| 35-39 36.042946438663826 37.0 37.0 37.0 37.0 37.0 | ||
| 40-44 36.02915462412439 37.0 37.0 37.0 37.0 37.0 | ||
| 45-49 35.98472630962014 37.0 37.0 37.0 37.0 37.0 | ||
| 50-54 35.95915958797262 37.0 37.0 37.0 37.0 37.0 | ||
| 55-59 35.93009967480203 37.0 37.0 37.0 37.0 37.0 | ||
| 60-64 35.90894747460392 37.0 37.0 37.0 37.0 37.0 | ||
| 65-69 35.877119399965125 37.0 37.0 37.0 37.0 37.0 | ||
| 70-74 35.83398706997363 37.0 37.0 37.0 37.0 37.0 | ||
| 75-79 35.8269856020124 37.0 37.0 37.0 37.0 37.0 | ||
| 80-84 35.81250209887877 37.0 37.0 37.0 37.0 37.0 | ||
| 85-89 35.787092952803874 37.0 37.0 37.0 37.0 37.0 | ||
| 90-94 35.749740143905115 37.0 37.0 37.0 37.0 37.0 | ||
| 95-99 35.69964211522571 37.0 37.0 37.0 37.0 37.0 | ||
| 100-104 35.66957645678475 37.0 37.0 37.0 37.0 37.0 | ||
| 105-109 35.611973979572284 37.0 37.0 37.0 37.0 37.0 | ||
| 110-114 35.589230799478955 37.0 37.0 37.0 37.0 37.0 | ||
| 115-119 35.515437915888256 37.0 37.0 37.0 37.0 37.0 | ||
| 120-124 35.458429493607824 37.0 37.0 37.0 37.0 37.0 | ||
| 125-129 35.41084434519564 37.0 37.0 37.0 37.0 37.0 | ||
| 130-134 35.354863307207424 37.0 37.0 37.0 37.0 37.0 | ||
| 135-139 35.342258299028984 37.0 37.0 37.0 37.0 37.0 | ||
| 140-144 35.27849923349239 37.0 37.0 37.0 37.0 37.0 | ||
| 145-149 35.20277554692679 37.0 37.0 37.0 29.8 37.0 | ||
| 150-151 34.985517090272005 37.0 37.0 37.0 25.0 37.0 | ||
| >>END_MODULE | ||
| >>Per sequence quality scores pass | ||
| #Quality Count | ||
| 2 6136.0 | ||
| 3 0.0 | ||
| 4 0.0 | ||
| 5 0.0 | ||
| 6 0.0 | ||
| 7 0.0 | ||
| 8 0.0 | ||
| 9 0.0 | ||
| 10 0.0 | ||
| 11 0.0 | ||
| 12 0.0 | ||
| 13 2.0 | ||
| 14 11.0 | ||
| 15 13.0 | ||
| 16 51.0 | ||
| 17 75.0 | ||
| 18 103.0 | ||
| 19 259.0 | ||
| 20 642.0 | ||
| 21 1778.0 | ||
| 22 4091.0 | ||
| 23 8947.0 | ||
| 24 16561.0 | ||
| 25 30598.0 | ||
| 26 51647.0 | ||
| 27 85815.0 | ||
| 28 134112.0 | ||
| 29 203904.0 | ||
| 30 287269.0 | ||
| 31 386418.0 | ||
| 32 486383.0 | ||
| 33 621980.0 | ||
| 34 845444.0 | ||
| 35 1617003.0 | ||
| 36 1.114483E7 | ||
| 37 2595523.0 | ||
| >>END_MODULE | ||
| >>Per sequence GC content fail | ||
| #GC Content Count | ||
| 0 7541.0 | ||
| 1 4029.0 | ||
| 2 585.0 | ||
| 3 767.5 | ||
| 4 1087.5 | ||
| 5 1336.5 | ||
| 6 1378.5 | ||
| 7 1310.0 | ||
| 8 1260.5 | ||
| 9 1196.5 | ||
| 10 1103.0 | ||
| 11 1069.0 | ||
| 12 2203.5 | ||
| 13 3331.0 | ||
| 14 2570.0 | ||
| 15 2232.0 | ||
| 16 2693.0 | ||
| 17 3801.0 | ||
| 18 8366.5 | ||
| 19 18257.5 | ||
| 20 31709.0 | ||
| 21 42520.0 | ||
| 22 37395.0 | ||
| 23 31781.5 | ||
| 24 42324.5 | ||
| 25 57243.5 | ||
| 26 70625.5 | ||
| 27 85643.0 | ||
| 28 102882.0 | ||
| 29 125409.0 | ||
| 30 164149.5 | ||
| 31 185493.0 | ||
| 32 176112.0 | ||
| 33 169254.5 | ||
| 34 170933.5 | ||
| 35 182841.0 | ||
| 36 183702.0 | ||
| 37 175507.0 | ||
| 38 183965.5 | ||
| 39 198382.0 | ||
| 40 239144.5 | ||
| 41 273772.0 | ||
| 42 279388.0 | ||
| 43 285036.5 | ||
| 44 301324.5 | ||
| 45 400213.0 | ||
| 46 607399.5 | ||
| 47 643191.5 | ||
| 48 699644.0 | ||
| 49 905352.0 | ||
| 50 1046961.5 | ||
| 51 1113562.0 | ||
| 52 1221545.5 | ||
| 53 1175322.0 | ||
| 54 952767.0 | ||
| 55 949484.5 | ||
| 56 940391.0 | ||
| 57 798618.0 | ||
| 58 638320.0 | ||
| 59 529596.0 | ||
| 60 400122.0 | ||
| 61 291239.0 | ||
| 62 236143.5 | ||
| 63 197968.5 | ||
| 64 219344.0 | ||
| 65 210411.5 | ||
| 66 179230.0 | ||
| 67 141792.0 | ||
| 68 101915.0 | ||
| 69 77625.5 | ||
| 70 57236.0 | ||
| 71 45750.0 | ||
| 72 43381.0 | ||
| 73 34811.5 | ||
| 74 24062.5 | ||
| 75 23394.0 | ||
| 76 25105.5 | ||
| 77 24670.0 | ||
| 78 20557.0 | ||
| 79 18035.5 | ||
| 80 15275.0 | ||
| 81 17289.0 | ||
| 82 31884.0 | ||
| 83 33557.0 | ||
| 84 19695.0 | ||
| 85 12132.0 | ||
| 86 6873.0 | ||
| 87 3657.5 | ||
| 88 3236.5 | ||
| 89 3459.0 | ||
| 90 2356.0 | ||
| 91 1126.0 | ||
| 92 549.0 | ||
| 93 300.0 | ||
| 94 168.5 | ||
| 95 114.0 | ||
| 96 82.5 | ||
| 97 50.5 | ||
| 98 34.5 | ||
| 99 31.0 | ||
| 100 21.5 | ||
| >>END_MODULE | ||
| >>Sequence Duplication Levels fail | ||
| #Total Deduplicated Percentage 20.30342204505982 | ||
| #Duplication Level Percentage of deduplicated Percentage of total | ||
| 1 73.44440888315017 14.911728304045383 | ||
| 2 15.41830464819508 6.260886929832246 | ||
| 3 4.672754285910515 2.846187071391099 | ||
| 4 1.9088954153405988 1.5502843703015972 | ||
| 5 1.0102485871903024 1.0255751718075061 | ||
| 6 0.6556698027614148 0.798740443659968 | ||
| 7 0.4458405097990209 0.6336461624663905 | ||
| 8 0.32830814126214586 0.5332623002299572 | ||
| 9 0.24340081769897368 0.44476825750694354 | ||
| >10 1.4752971841946068 5.722577077584334 | ||
| >50 0.16076080116660646 2.2846194640256967 | ||
| >100 0.15378571894069337 6.713655148751547 | ||
| >500 0.030938832731614126 4.4216044214127495 | ||
| >1k 0.03812884979271724 16.932260611564846 | ||
| >5k 0.007652715873665971 10.916805907389325 | ||
| >10k+ 0.005604805991980712 24.003398358030616 | ||
| >>END_MODULE | ||
| >>Overrepresented sequences warn | ||
| #Sequence Count Percentage Possible Source | ||
| GTTTGTTTTTGACCGTTTTGTGATCAAGGGTGTTGAGGCGAGGGCTCCTA 91363 0.49306528286236156 No Hit | ||
| GCCGTATTTACAGCGCCAGTGGGCGTACTGATCTTGAAGACAATGGTGTT 80212 0.43288587796980993 No Hit | ||
| GTTGAGGCGAGGGCTCCTAGAGTCCTGCTGGCGATAAGCTTACGTGAGAA 69138 0.37312202452347176 No Hit | ||
| GTTGGTGTTTCCAAGTCCGGTAAGCACATTGACGTTTGTGCCGCCTGTTG 67757 0.3656690823517729 No Hit | ||
| CCTGGATCTACTGAAAAGTCGGGAGCTGCAAAGGCGCATTTTAAAAACGC 66088 0.3566618698357951 No Hit | ||
| CCGGGATGTGCTGGACGGCTCCAACAACTTGCGTTAAAAGGCGTTTTACA 60807 0.3281615167519851 No Hit | ||
| CCCGGGATGTGCTGGACGGCTCCAACAACTTGCGTTAAAAGGCGTTTTAC 60630 0.32720628810289704 No Hit | ||
| GTTGTGTTGCAATCCTTGATGCTAAGGGTACGCCCGTGGAAATCGTCGGG 57945 0.31271595520571277 No Hit | ||
| CTCGTTTCTCTGCGTTCGATCACTTTCTCTATAATTTTCGACCGGGACTG 57664 0.31119946226563505 No Hit | ||
| CCAGAATTTTGCATTATCGCGGGCTGGGACGGCAACTGGGAATCTCATCT 51042 0.27546203789127605 No Hit | ||
| CTCACGTGCACTGCTTACGCAGATGCATGGACTACGGCAGTAGTCGTAGT 50334 0.2716411232949236 No Hit | ||
| GTCGGTTCCAATGGGCACTTCAGTACGGAAATAAGCGAAACCGGGTACTG 49381 0.2664979995515282 No Hit | ||
| GGCGTTTTACAGTTTCCCAGAATTTTGCATTATCGCGGGCTGGGACGGCA 48201 0.2601298085576075 No Hit | ||
| GTCGTCTTGTGTTTCAGCAAGAGTCAGGTCAACTCGCCAGACTTGCACTG 47552 0.256627303510951 No Hit | ||
| GTCGAACTCAGGAGATACTCCACTGAATTGGAACAACGACGAGTCAGTGT 46608 0.2515327507158144 No Hit | ||
| GTTGGTGCGGCGGGTTGTACCTTGCTCACGTGCACTGCTTACGCAGATGC 45178 0.2438153667147069 No Hit | ||
| GGTGTTTCCAAGTCCGGTAAGCACATTGACGTTTGTGCCGCCTGTTGGTT 43513 0.23482974128684408 No Hit | ||
| CTTGTGTTTCAGCAAGAGTCAGGTCAACTCGCCAGACTTGCACTGAACCG 42299 0.22827806004394594 No Hit | ||
| GGCTCATACAACACGTTGACTTGTCCCGCCGTCATGCACTTACGCTTTAG 42142 0.22743076683543273 No Hit | ||
| CCCGGATTGACCTTTCGCTTTCCGCAGCCGCCGAGGGGCTGCACCAGTGC 41256 0.22264922681796334 No Hit | ||
| GTCAGATCTTTAGCGAAATAAAAATACCGGTTTCCTCTTTCGATGTCGTG 40766 0.2200048085238776 No Hit | ||
| GTCCACGTGAGTTTCCGTTAGAGACGTCTTGTAAGTAACGTGCTGCCGTG 40754 0.21994004725953265 No Hit | ||
| CCCAGTTCCAATCATTCCGACTGGCCCCGGGATGTGCTGGACGGCTCCAA 38576 0.2081858777809229 No Hit | ||
| CTTCATTCGTGCAGTTATCGCTGCCAGCGTCCGTCCCGTGGTCTCTACAG 37594 0.2028862476486939 No Hit | ||
| GTTCAAGGAAAATTATTCGGCGATGTGGGTCACCCTGGATACACTTCTGT 35087 0.18935654017262654 No Hit | ||
| GCCAGAATAATTATCGCGAGGTGCTAATGACCTTACGCCGGGCAGACCGT 34743 0.18750005059473776 No Hit | ||
| GCGTTTTACAGTTTCCCAGAATTTTGCATTATCGCGGGCTGGGACGGCAA 34665 0.18707910237649555 No Hit | ||
| CCGGTTTCCTCTTTCGATGTCGTGGTTACTCATTGACACATTGTAAGGCT 33373 0.18010647291535514 No Hit | ||
| GTGAGCGTTTGACAACTTGTACTGCTCCTCACGGGTGGCCCCCTTCACCT 33196 0.179151244266267 No Hit | ||
| GTTGTACCTTGCTCACGTGCACTGCTTACGCAGATGCATGGACTACGGCA 33101 0.17863855092353612 No Hit | ||
| GGTTGGATGAGTGAGTAGAGACAGTGGCGGCTACCATGTTGATGCGTGCT 32865 0.17736491272475194 No Hit | ||
| CCGGGACTGGGCGTCATATGGCTTACACAAGCGTATCTTCACCAAACTCT 32414 0.1749309685397873 No Hit | ||
| CCTCTTTCGATGTCGTGGTTACTCATTGACACATTGTAAGGCTCGTATCC 30918 0.16685739758478263 No Hit | ||
| CCGACATCGAACTGAGGTAAATTCGAACCTTCAGAGCCGTGGTGCGCACG 30917 0.16685200081275386 No Hit | ||
| CTGCACTTCACGCACTGCTGCCGTATCCCATGGATGGCCGACGCCAGGAT 30253 0.16326854418566622 No Hit | ||
| GTGGTGATGTTGCGCGGATCATTCGTCGCTGCATATGCCTCAGCCTTGAT 30221 0.16309584748074635 No Hit | ||
| GTCGGGGATCCCTTTACCTGGATCTACTGAAAAGTCGGGAGCTGCAAAGG 29014 0.1565819436420494 No Hit | ||
| CCCGTGGTCTCTACAGTGTGGCGTGAGCCATTCGCCGACAAAGATATTTT 29003 0.15652257914973317 No Hit | ||
| GGTGAATTGAATTGATGTTGTGTTGCAATCCTTGATGCTAAGGGTACGCC 28091 0.1516007230595164 No Hit | ||
| GCTGGGACGGCAACTGGGAATCTCATCTTCAGGTCGTGGTAGGCTCGAAG 27980 0.15100168136432554 No Hit | ||
| CTCATACAACACGTTGACTTGTCCCGCCGTCATGCACTTACGCTTTAGGC 27915 0.15065089118245703 No Hit | ||
| TGGGACGGCAACTGGGAATCTCATCTTCAGGTCGTGGTAGGCTCGAAGCG 27325 0.14746679568549664 No Hit | ||
| GCTCATACAACACGTTGACTTGTCCCGCCGTCATGCACTTACGCTTTAGG 27081 0.14614998331048248 No Hit | ||
| CCGTGGTCTCTACAGTGTGGCGTGAGCCATTCGCCGACAAAGATATTTTA 26771 0.1444769839815711 No Hit | ||
| GTTGGATGAGTGAGTAGAGACAGTGGCGGCTACCATGTTGATGCGTGCTT 26656 0.14385635519826526 No Hit | ||
| CCCCAACCGGCTTCATACCTTACACCAGCAGTCGTCGTGCCCATTTGCAT 26473 0.14286874591700466 No Hit | ||
| GTTGCAATCCTTGATGCTAAGGGTACGCCCGTGGAAATCGTCGGGGATCC 26246 0.14164367866647923 No Hit | ||
| GTCGCTTTGTCCGTGGCTAAGTATCCATGAGCCCTATTCGCCGCCGCCTG 26166 0.1412119369041795 No Hit | ||
| GTCTGATTGTATTGGTCAGGCTGTTACTGAGCACCCACTCCCGGCCGCTA 25657 0.13846497994154755 No Hit | ||
| GTGAGAAGTTGGTGCAGCCCAGTTCCAATCATTCCGACTGGCCCCGGGAT 25506 0.13765006736520685 No Hit | ||
| CCTCGTTTCTCTGCGTTCGATCACTTTCTCTATAATTTTCGACCGGGACT 25104 0.13548056500965078 No Hit | ||
| GTCCAGCAACGCCGGTTCCAGCCCACTTTCATGTGCCAGAACTGCATCAT 25003 0.1349354910347474 No Hit | ||
| GGCGTACTGATCTTGAAGACAATGGTGTTGGTGTTTCCAAGTCCGGTAAG 24618 0.13285773380368 No Hit | ||
| GAGCGTTTGACAACTTGTACTGCTCCTCACGGGTGGCCCCCTTCACCTTA 23785 0.1283622227037342 No Hit | ||
| GGATGAGTGAGTAGAGACAGTGGCGGCTACCATGTTGATGCGTGCTTTTT 23722 0.1280222260659232 No Hit | ||
| GGTGATGTTGCGCGGATCATTCGTCGCTGCATATGCCTCAGCCTTGATAA 23497 0.12680795235945524 No Hit | ||
| TGGTGATGTTGCGCGGATCATTCGTCGCTGCATATGCCTCAGCCTTGATA 23447 0.1265381137580179 No Hit | ||
| GGATGTTGGTGCGGCGGGTTGTACCTTGCTCACGTGCACTGCTTACGCAG 23420 0.12639240091324178 No Hit | ||
| GCCATCAGTAGTTATTGGGCTTCCACTCCGGGTGCCCCAACCGGCTTCAT 22845 0.12328925699671256 No Hit | ||
| CCCAAGTTCTTCACTACGTCCAGCAACGCCGGTTCCAGCCCACTTTCATG 22642 0.12219371227487703 No Hit | ||
| CTCGTCTAGCCCAAGTGCCGATGATCGGAGTCGCTTTGTCCGTGGCTAAG 22458 0.12120070622158767 No Hit | ||
| CCTTATACTCCTTAAACGGGATGACCGTGTTGGCGGGCCTCTTGACCCGA 22167 0.11963024556122245 No Hit | ||
| GTGGATTTGTCAAATGCGTAAGTGTAAGCACCGTCGATAAAGTTGCCAGA 22059 0.11904739418211786 No Hit | ||
| CCCCGGGATGTGCTGGACGGCTCCAACAACTTGCGTTAAAAGGCGTTTTA 21869 0.11802200749665603 No Hit | ||
| GGGTGTTGAGGCGAGGGCTCCTAGAGTCCTGCTGGCGATAAGCTTACGTG 21865 0.11800042040854104 No Hit | ||
| GTAAATTCGAACCTTCAGAGCCGTGGTGCGCACGTGTTAAACCGACCGCA 21775 0.11751471092595386 No Hit | ||
| CCTGCACTTCACGCACTGCTGCCGTATCCCATGGATGGCCGACGCCAGGA 21491 0.11598202766978988 No Hit | ||
| GGTGTTTGTTTTTGACCGTTTTGTGATCAAGGGTGTTGAGGCGAGGGCTC 21392 0.11544774723894398 No Hit | ||
| CCTTGCTCACGTGCACTGCTTACGCAGATGCATGGACTACGGCAGTAGTC 20833 0.11243095167487471 No Hit | ||
| GTGAGTTTCCGTTAGAGACGTCTTGTAAGTAACGTGCTGCCGTGACGAAT 20796 0.11223127110981108 No Hit | ||
| GGTCTCTACAGTGTGGCGTGAGCCATTCGCCGACAAAGATATTTTATCGT 20699 0.11170778422302269 No Hit | ||
| CTCATCGGTAAAAGCGCCGGGGCTGCAGCCAAGGCGGTGGTCACAGCATG 20627 0.11131921663695293 No Hit | ||
| CTCATGGTTAGGTTCCAGTGTCTCAACACGAGGCCCGGGTCCGACCACCT 20590 0.11111953607188933 No Hit | ||
| CCCTCACGCAGATAGTGTCGCCAGTGTAATCCCATAGCTGATGACTGTAG 20450 0.11036398798786483 No Hit | ||
| CTGGGACGGCAACTGGGAATCTCATCTTCAGGTCGTGGTAGGCTCGAAGC 20134 0.10865860802678094 No Hit | ||
| GCCACGTCTGAATTGAACCCCCTCATCGGTAAAAGCGCCGGGGCTGCAGC 20123 0.10859924353446473 No Hit | ||
| CCTGCTTCGGCGTTTTCCCAGGGCCGTACCACGGCTGCTCGTATAAGATC 20058 0.10824845335259622 No Hit | ||
| CTGCGTTCGATCACTTTCTCTATAATTTTCGACCGGGACTGGGCGTCATA 20058 0.10824845335259622 No Hit | ||
| CTAATATGTAAGTGTCAGTGCCAGGGGTGAATTGAATTGATGTTGTGTTG 19702 0.10632720251036248 No Hit | ||
| CCTCACGCAGATAGTGTCGCCAGTGTAATCCCATAGCTGATGACTGTAGC 19628 0.10592784138023525 No Hit | ||
| GTCCCGTGGTCTCTACAGTGTGGCGTGAGCCATTCGCCGACAAAGATATT 19211 0.10367738744424798 No Hit | ||
| GTCTTGTGTTTCAGCAAGAGTCAGGTCAACTCGCCAGACTTGCACTGAAC 19194 0.10358564231975928 No Hit | ||
| CTTGGCTTCGACTTTAAGGCCCAAGTTCTTCACTACGTCCAGCAACGCCG 19168 0.10344532624701189 No Hit | ||
| CCGGCATCTGGTCCAGGGCTTTGGCTTCACTGACAGCTTTATCCAAAGCC 19092 0.10303517157282714 No Hit | ||
| GTTCGGCTAAGAGTCCACGTGAGTTTCCGTTAGAGACGTCTTGTAAGTAA 19079 0.10296501353645345 No Hit | ||
| CCCAGGGCCGTACCACGGCTGCTCGTATAAGATCTTCTCTTTAAAAGCAT 18774 0.10131899806768577 No Hit | ||
| >>END_MODULE |