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DESeqDataSetFromTximport requires both counts and effective length. While PISCES uses effective length internally for DESeq2, it does not write out the values for use in downstream analysis.
The text was updated successfully, but these errors were encountered:
@carloravagli I see that I should add the weighted gene-level effective length matrices as well. The tximport package provides summarizeToGene() for this purpose, which internally weights the lengths by abundance and takes an average:
It can replace any problematic rows with a simple geometric mean as well. PISCES uses tximport internally so I will just write out the one extra gene-level length matrix.
DESeqDataSetFromTximport
requires both counts and effective length. While PISCES uses effective length internally for DESeq2, it does not write out the values for use in downstream analysis.The text was updated successfully, but these errors were encountered: