Added second image difference function that subtracts the images with…

…out the 32 bit option.

OSOV Toolbox.ijm

@@ -18,6 +18,7 @@ requires("1.50a");
 	var filemenu = newMenu("OSOV Menu Tool", newArray(
 		"Colour Slices",
 		"Image Difference",
+		"Image Difference v2",
 //	"Image Difference (batch)",
 		"Clear Slices",
 		"Save Slices",
@@ -42,6 +43,7 @@ requires("1.50a");
 		if (menuCmd!="-") {
 			if (menuCmd=="Colour Slices") { run("OSOV Colour Slices"); }
 			else if (menuCmd=="Image Difference") { run("OSOV Image Difference"); }
+			else if (menuCmd=="Image Difference v2") { run("OSOV Image Difference v2"); }
 			else if (menuCmd=="Image Difference (batch)") { run("OSOV Batch Image Difference");}
 			else if (menuCmd=="Clear Slices") { run("OSOV Clear Slices"); }
 			else if (menuCmd=="Save Slices") { run("OSOV Save Slices"); }

OSOV/OSOV_Image_Difference_v2.ijm

@@ -0,0 +1,45 @@
+var SAMPLE_TYPE_STEM = "Stem";
+var SAMPLE_TYPE_LEAF = "Leaf";
+
+var sampleType;
+
+macro "OSOV Image Difference v2" {
+
+	getSettings();
+
+	setBatchMode(true);
+
+	originalImage = getImageID();
+
+	Stack.getDimensions(ww, hh, channels, slices, frames);
+
+	run("Make Substack..."," slices=1-"+ slices-1);
+	imgID1 = getImageID();
+
+	selectImage(originalImage);
+
+	run("Make Substack...", " slices=2-"+ slices);
+	imgID2 = getImageID();
+
+	if(sampleType == SAMPLE_TYPE_STEM) {
+		imageCalculator("Subtract create stack", imgID2, imgID1);
+	} else {
+		imageCalculator("Subtract create stack", imgID1, imgID2);
+	}
+	selectImage(imgID1);
+	close();
+
+	selectImage(imgID2);
+	close();
+
+	setBatchMode("exit and display");
+}
+
+
+
+function getSettings() {
+	Dialog.create("Difference Settings");
+	Dialog.addChoice("Sample:", newArray(SAMPLE_TYPE_STEM, SAMPLE_TYPE_LEAF));
+	Dialog.show();
+	sampleType = Dialog.getChoice();
+}