PMID:30727143 Baseline Sensitivity of Monilinia fructicola from China to the DMI Fungicide SYP-Z048 and Analysis of DMI-Resistant Mutants

[jingluodatacurator]

Curated by @jingluodatacurator

https://canto.phi-base.org/curs/374b149aa1776aac (this session will need to be deleted)

Link with correct PMID https://canto.phi-base.org/curs/93b1e80c305cc8da

Uniprot No.:D0VVD6
Strains: MBJA8, MSB11, MPA18
Main expt. for curation: see in Materials and Methods 'Generation of SYP-Z048-resistant mutants' section.

[jingluodatacurator]

Hi @CuzickA, the paper doesn't allow editing unfortunately.

[CuzickA]

Hi @jingluodatacurator, I'm looking to see if there is an alternative name for 'SYP-Z048 (5-(4-chlorophenyl)-2,3-di-methyl-3-(pyridine-3)-oxazoline) '

It looks like 'pyrisoxazole' could be a synonym

Do you agree that this chemical is 'pyrisoxazole'?

(Note: pyrisoxazole is on the FRAC list and already added to our anti-infective list).

[CuzickA]

Mutant generation by UV irradiation.
'Mutants of M. fructicola were not selected spontaneously in the laboratory on YGA medium amended with SYP-Z048 in this study. The fungus had to be exposed to UV irradiation in order to gener-ate SYP-Z048-resistant mutants. '

It is not clear to me from the paper which parental strains generated which UV mutants. I think we will have to choose a representative wild type S strain and a R mutant. Several S and R strains had Y136F respectively so hopefully this will rule out complications of any other UV mutations in the background outside of the Cyp51 target.

[CuzickA]

Current annotation

I think we need to change the strain here to 'MPA18 ' to represent the S wildtype isolate in Table 4. For some reason strain 'MBJA8' is not mentioned in Table 4, just in earlier Tables.

@jingluodatacurator, please could you read my comments above and see if you agree. If you do, please could you add the strain 'MPA18' instead of 'MBJA8'

[CuzickA]

Added AE alteration_in_archetype

M. fructicola CYP51(aaY136F)[Not assayed]

Y137F; Cyp51 (Cyp51B); SEPTTR

Straight forward to find in Nichola's S file in Table S2

[CuzickA]

Next steps

1. @jingluodatacurator to check AC's notes on chemical name. now DONE and agreed
2. @jingluodatacurator to check AC's comments and change strain name if in agreement. DONE 12_12_2023
3. AC to add new PHIPO and PHI-ECO terms. now DONE 05_12_2023
4. AC to check final edits (points 1-3). DONE 12_12_2023
5. AC to check AE alteration in archetype with Nichola.
6. @jingluodatacurator recreate the session using the correct PMID (see comment below 12_12_2023). DONE 12_12_2023

Then session will be ready for approval.

[jingluodatacurator]

Hi @jingluodatacurator, I'm looking to see if there is an alternative name for 'SYP-Z048 (5-(4-chlorophenyl)-2,3-di-methyl-3-(pyridine-3)-oxazoline) '

It looks like 'pyrisoxazole' could be a synonym

Do you agree that this chemical is 'pyrisoxazole'?

(Note: pyrisoxazole is on the FRAC list and already added to our anti-infective list).

@CuzickA, yes it looks correct to me.

[CuzickA]

Hi @jingluodatacurator, I have just noticed that the PMID and title entered here for curation does not match the title of the paper that we are curating.

I have just looked up the correct PMID for this article and it is PMID: 30727143 not PMID:30717143.

Please could you start a new curation session with the correct PMID and enter the curated information. Unfortunately there is no shortcut for this but at least there is only one annotation :-). (Once this is done, I will then ask James to delete the incorrect PMID:30717143 session from PHI-Canto.)

Here is the UniProt D0VVD6 and if you agree with my comments above you can amend the strain in the genotype from 'MBJA8' to 'MPA18'.

[jingluodatacurator]

Hi @jingluodatacurator, I have just noticed that the PMID and title entered here for curation does not match the title of the paper that we are curating.

I have just looked up the correct PMID for this article and it is PMID: 30727143 not PMID:30717143.

Please could you start a new curation session with the correct PMID and enter the curated information. Unfortunately there is no shortcut for this but at least there is only one annotation :-). (Once this is done, I will then ask James to delete the incorrect PMID:30717143 session from PHI-Canto.)

Here is the UniProt D0VVD6 and if you agree with my comments above you can amend the strain in the genotype from 'MBJA8' to 'MPA18'.

Hi @CuzickA, thanks so much for spotting the mistake (I was wondering why I couldn't find the curation session in my curation list).

[jingluodatacurator]

Hi @CuzickA, I have created the new session with annotations, please check.

https://canto.phi-base.org/curs/93b1e80c305cc8da

[CuzickA]

Hi @jingluodatacurator, thank you for doing this. I have just made a couple of changes to match the original entry.

[CuzickA]

Next steps

1. AC to check AE alteration in archetype with Nichola.

Then session will be ready for approval.

2. AC to add Monilinia fructicola strain 'MPA18' to strain list. DONE 08_01_2024
3. AC to ask James to delete incorrect PMID/session PMID:30717143 / https://canto.phi-base.org/curs/374b149aa1776aac

[CuzickA]

Hi @jseager7, please could you delete this incorrectly added PMID/session from PHI-Canto?

AC to ask James to delete incorrect PMID/session PMID:30717143 / https://canto.phi-base.org/curs/374b149aa1776aac

[jseager7]

Hi jseager7, please could you delete this incorrectly added PMID/session from PHI-Canto?

Sure, I'll try to get this done this week.

[jseager7]

PMID:30717143 and its curation session (374b149aa1776aac) have been removed from PHI-Canto now.

[CuzickA]

AE alteration in archetype checked by Nichola and looks fine.

Approving session and closing ticket.