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Whole genome BSseq December 2015

kubu4 edited this page Oct 3, 2018 · 9 revisions

Progress of high-throughput sequencing, and analysis, of Olympia oyster (Ostrea lurida) bisulfite-treated DNA from Fidalgo Bay broodstock offspring, grown out at two locations:

  • Fidalgo Bay (1NF)
  • Oyster Bay (2NF)
Notebook(s):
Discussions:
Sample collection date(s), sample tissue(s), storage method, storage location, personnel:
Sample(s):
  • NF2_6 (Lotterhos project - described elsewhere)
  • NF_18 (Lotterhos project - described elsewhere)
  • 1NF11
  • 1NF15
  • 1NF16
  • 1NF17
  • 2NF5
  • 2NF6
  • 2NF7
  • 2NF8
Sample name explanations:

1NF#

1 = Fidalgo Bay outplants

NF = Fidalgo Bay broodstock origination

# = Sample number

2NF#

Same as above, but:

2 = Oyster Bay outplants

DNA extraction date(s), tissue(s), method(s), and personnel:
Library construction date(s) and personnel:

Sequencing Details

Sequencing: GENEWIZ, Inc.
  • HiSeq2500
  • Single lane
  • 50bp SE, single-index
Sample submission date(s):
QC Report(s) and brief summaries:
Data receipt date(s), format(s), description, and storage location:

Received: 20160108

Format: FASTQ

Location: http://owl.fish.washington.edu/nightingales/O_lurida/

Files:

FILENAME READS SAMPLE
11_GGCTAC_L001_R1_001.fastq.gz 10933121 NF2_6
12_CTTGTA_L001_R1_001.fastq.gz 10816647 NF_18
1_ATCACG_L001_R1_001.fastq.gz 9402890 1NF11
2_CGATGT_L001_R1_001.fastq.gz 11954873 1NF15
3_TTAGGC_L001_R1_001.fastq.gz 11817358 1NF16
4_TGACCA_L001_R1_001.fastq.gz 11606618 1NF17
5_ACAGTG_L001_R1_001.fastq.gz 12589609 2NF5
6_GCCAAT_L001_R1_001.fastq.gz 12489766 2NF6
7_CAGATC_L001_R1_001.fastq.gz 10295293 2NF7
8_ACTTGA_L001_R1_001.fastq.gz 14374642 2NF8
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