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Describe provenance of Geoduck RNA-seq on NCBI SRA #1247

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sr320 opened this issue Jun 30, 2021 · 14 comments
Closed

Describe provenance of Geoduck RNA-seq on NCBI SRA #1247

sr320 opened this issue Jun 30, 2021 · 14 comments
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@sr320
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sr320 commented Jun 30, 2021

Thinking about geoduck RNAseq (NS and Hiseq) provide a description of the relationship of
https://sr320.github.io/Illumina-Summary/

to what is on SRA - (Notebook post is fine and likely already exists :)

@kubu4
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kubu4 commented Jul 8, 2021

Notebook entry is here:

https://robertslab.github.io/sams-notebook/2016/12/21/sample-submission-geoduck-tissue-gdna-for-illumina-pilot-sequencing-project.html

The TL;DR is that we sent tissue to Illumina and they did everything else. All tissue from single geoduck.

@sr320
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sr320 commented Jul 8, 2021

Thanks! I am looking for how to match these with SRAs

@kubu4
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kubu4 commented Jul 8, 2021

I don't think I understand fully what you're looking for. The info is in nightingales spreadsheet.

@kubu4
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kubu4 commented Jul 8, 2021

image

@kubu4
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kubu4 commented Jul 8, 2021

The last column in the screencap above is the SRA BioProject accession.

@kubu4
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kubu4 commented Jul 8, 2021

Sorry for being a dolt (still getting my head into "work mode"). I just realized what you're looking for! BRB...

@kubu4
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kubu4 commented Jul 8, 2021

Do you want accessions which lead to this NCBI page (NCBI "Run"):

image

or do you want accessions which lead to this NCBI page (NCBI "Accession"):

image

@kubu4
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kubu4 commented Jul 8, 2021

Or, this NCBI page (NCBI "Run Selector"):

image

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kubu4 commented Jul 8, 2021

Now that I've posted all that above, I'll just put together a notebook which links to each one! How about that??!! :)

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kubu4 commented Jul 12, 2021

New notebook entry is below. Let me know if you want me to make any changes.

https://robertslab.github.io/sams-notebook/2021/07/12/Summary-Geoduck-RNAseq-Data.html

@sr320
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sr320 commented Jul 12, 2021

So cross-referencing with https://sr320.github.io/Illumina-Summary/

looks like

NR-21-Larvae-d5
and
Geoduck-larvae-day5-RNA-EPI-99

are not in SRA?

@kubu4
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kubu4 commented Jul 12, 2021

They are. I just neglected to incorporate them... Sorry!

@sr320
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sr320 commented Jul 12, 2021 via email

@kubu4
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kubu4 commented Jul 12, 2021

Update has been pushed. Might take a few mins to update.

@sr320 sr320 closed this as completed Aug 10, 2021
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