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Updated README with info on base transcriptomes
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Mike Stubbington committed Sep 26, 2018
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Expand Up @@ -141,8 +141,7 @@ Type of sequence to be analysed. Since TraCeR currently only works with TCR sequ
Mmus = /path/to/kallisto/transcriptome_for_Mmus
Hsap = /path/to/kallisto/transcriptome_for_Hsap

Location of the transcriptome fasta file to which the specific TCR sequences will be appended from each cell. Can be downloaded from http://bio.math.berkeley.edu/kallisto/transcriptomes/ and many other places. This must be a plain-text fasta file so decompress it if necessary (files from the Kallisto link are gzipped).

Location of the transcriptome fasta file to which the specific TCR sequences will be appended from each cell. Instructions on how to make reference transcriptomes can be found at: http://www.nxn.se/valent/2016/10/3/the-first-steps-in-rna-seq-expression-analysis-single-cell-and-other. Reference trasncriptomes should be uncompressed fasta files.
#### Base indices for Kallisto/Salmon
[salmon_base_indices]
Mmus = /path/to/salmon/index_for_Mmus
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