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I have received this error message when aligning single-end Illumina reads.
EXITING because of FATAL ERROR in reads input: quality string length is not equal to sequence length
@HISEQ:206:HVK53ADXX:1:2109:20411:84904
TGGAAAATGAAACCGATGAGAGAGAACTGGATTTCACCCACGCTGGCAATTGCCACGCCGAAGACGATGAGCAAGA
CCCFFFFFHHHHHJJIIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHFDDDDDDDDDDDDDDDC
SOLUTION: fix your fastq file
I have checked the FASTQ file and the read which is referred to in the error message is:
@HISEQ:206:HVK53ADXX:1:2109:20411:84904 1:N:0:ATCACG
TGGAAAATGAAACCGATGAGAGAGAACTGGATTTCACCCACGCTGGCAATTGCCACGCCGAAGACGATGAGCAAGA
+
CCCFFFFFHHHHHJJIIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHFDDDDDDDDDDDDDDDC
Sequence and quality string (2nd and 4th lines) have the same length (77).
When I modified my FASTQ file by removing the description field all ID lines (after space), STAR works. The original read names are:
@HISEQ:206:HVK53ADXX:1:2109:20411:84904 1:N:0:ATCACG
An I convert this to:
@HISEQ:206:HVK53ADXX:1:2109:20411:84904
this is likely caused by some formatting problem at the end of the read name file, e.g. an extra space at the end, or some other invisible control character.
I have received this error message when aligning single-end Illumina reads.
EXITING because of FATAL ERROR in reads input: quality string length is not equal to sequence length
@HISEQ:206:HVK53ADXX:1:2109:20411:84904
TGGAAAATGAAACCGATGAGAGAGAACTGGATTTCACCCACGCTGGCAATTGCCACGCCGAAGACGATGAGCAAGA
CCCFFFFFHHHHHJJIIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHFDDDDDDDDDDDDDDDC
SOLUTION: fix your fastq file
I have checked the FASTQ file and the read which is referred to in the error message is:
@HISEQ:206:HVK53ADXX:1:2109:20411:84904 1:N:0:ATCACG
TGGAAAATGAAACCGATGAGAGAGAACTGGATTTCACCCACGCTGGCAATTGCCACGCCGAAGACGATGAGCAAGA
+
CCCFFFFFHHHHHJJIIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHFDDDDDDDDDDDDDDDC
Sequence and quality string (2nd and 4th lines) have the same length (77).
I attach the log files. This is from STAR 2.6.1c
sample_Log.txt
But the same occurs when I tried with 2.7.1a and 2.7.2b.
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