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lcoombe committed Jun 22, 2018
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### ARCS+LINKS Pipeline

ARCS requires two input files:
* Draft assembly fasta file
* Interleaved linked reads file (Barcode sequence expected in the BX tag of the read header or in the form "@readname_barcode" ; Run [Long Ranger basic](https://support.10xgenomics.com/genome-exome/software/pipelines/latest/what-is-long-ranger) on raw chromium reads to produce this interleaved file)

The Makefile located here: Examples/arcs-make will run the full ARCS pipeline. It will also optionally run the misassembly corrector [Tigmint](https://github.com/bcgsc/tigmint) prior to scaffolding with ARCS.

There are three steps to the pipeline:

1. Run ARCS to generate a Graphviz Dot file (.gv). Nodes in the graph are the sequences to scaffold, and edges show that there is evidence to suggest nodes are linked based on the data obtained from the GemCode/Chromium reads.
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