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Enveomics collection added as submodule
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lmrodriguezr committed Jun 4, 2017
1 parent f6a1c6a commit 4b7a53e
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Showing 7 changed files with 26 additions and 23 deletions.
3 changes: 3 additions & 0 deletions .gitmodules
@@ -0,0 +1,3 @@
[submodule "utils/enveomics"]
path = utils/enveomics
url = https://github.com/lmrodriguezr/enveomics.git
2 changes: 1 addition & 1 deletion miga-base.gemspec
Expand Up @@ -19,7 +19,7 @@ Gem::Specification.new do |s|
# Files # Files
s.files = Dir[ s.files = Dir[
"lib/**/*.rb", "test/**/*.rb", "lib/miga/_data/**/*", "lib/**/*.rb", "test/**/*.rb", "lib/miga/_data/**/*",
"scripts/*.bash", "utils/*", "bin/*", "actions/*", "scripts/*.bash", "utils/**/*", "bin/*", "actions/*",
"Gemfile", "Rakefile", "README.md", "LICENSE" "Gemfile", "Rakefile", "README.md", "LICENSE"
] ]
s.executables << "miga" s.executables << "miga"
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7 changes: 4 additions & 3 deletions scripts/trimmed_fasta.bash
Expand Up @@ -21,13 +21,14 @@ for sis in 1 2 ; do
done done


# FastQ -> FastA # FastQ -> FastA
FastQ.toFastA.awk < "../02.trimmed_reads/$b.1.clipped.fastq" > "$b.1.fasta" FQ2A="$MIGA/utils/enveomics/Scripts/FastQ.toFastA.awk"
awk -f "$FQ2A" < "../02.trimmed_reads/$b.1.clipped.fastq" > "$b.1.fasta"
if [[ -e "../02.trimmed_reads/$b.2.clipped.fastq" ]] ; then if [[ -e "../02.trimmed_reads/$b.2.clipped.fastq" ]] ; then
FastQ.toFastA.awk < "../02.trimmed_reads/$b.2.clipped.fastq" > "$b.2.fasta" awk -f "$FQ2A" < "../02.trimmed_reads/$b.2.clipped.fastq" > "$b.2.fasta"
FastA.interpose.pl "$b.CoupledReads.fa" "$b".[12].fasta FastA.interpose.pl "$b.CoupledReads.fa" "$b".[12].fasta
gzip -9 -f "$b.2.fasta" gzip -9 -f "$b.2.fasta"
gzip -9 -f "$b.1.fasta" gzip -9 -f "$b.1.fasta"
FastQ.toFastA.awk < "../02.trimmed_reads/$b".[12].clipped.single.fastq \ awk -f "$FQ2A" < "../02.trimmed_reads/$b".[12].clipped.single.fastq \
> "$b.SingleReads.fa" > "$b.SingleReads.fa"
gzip -9 -f "$b.SingleReads.fa" gzip -9 -f "$b.SingleReads.fa"
else else
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1 change: 1 addition & 0 deletions utils/enveomics
Submodule enveomics added at 64607e
Empty file modified utils/index_metadata.rb 100644 → 100755
Empty file.
Empty file modified utils/plot-taxdist.R 100644 → 100755
Empty file.
36 changes: 17 additions & 19 deletions utils/requirements.txt
@@ -1,19 +1,17 @@
Software Test executable Website Notes Software Test exec Website Notes
-------- --------------- ------- ----- -------- --------- ------- -----
Enve-omics scripts FastQ.tag.rb http://github.com/lmrodriguezr/enveomics All the collection must be present R R http://www.r-project.org/
SolexaQA++ SolexaQA++ http://solexaqa.sourceforge.net Required version: v3.1.3+ SQLite3 sqlite3 https://www.sqlite.org/
Scythe scythe https://github.com/vsbuffalo/scythe Required version: 0.991+ NCBI BLAST+ blastp ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST
FastQC fastqc http://www.bioinformatics.babraham.ac.uk/projects/fastqc HMMer 3.0+ hmmsearch http://hmmer.janelia.org/software
IDBA idba_ud http://i.cs.hku.hk/~alse/hkubrg/projects/idba Bedtools bedtools http://bedtools.readthedocs.org/en/latest/
Prodigal prodigal http://prodigal.ornl.gov Prodigal prodigal http://prodigal.ornl.gov
HMMer 3.0+ hmmsearch http://hmmer.janelia.org/software IDBA idba_ud http://i.cs.hku.hk/~alse/hkubrg/projects/idba
NCBI BLAST+ blastp ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST MCL mcl http://micans.org/mcl/
R R http://www.r-project.org/ Barrnap barrnap http://www.vicbioinformatics.com/software.barrnap.shtml
SQLite3 sqlite3 https://www.sqlite.org/ Scythe scythe https://github.com/vsbuffalo/scythe Required version: 0.991+
RAxML (pthreads) raxmlHPC-PTHREADS http://sco.h-its.org/exelixis/web/software/raxml/index.html FastQC fastqc http://www.bioinformatics.babraham.ac.uk/projects/fastqc
MCL mcl http://micans.org/mcl/ SolexaQA++ SolexaQA++ http://solexaqa.sourceforge.net Required version: v3.1.3+
DIAMOND diamond http://ab.inf.uni-tuebingen.de/software/diamond Required version: v0.7.9+ DIAMOND (opt) diamond http://ab.inf.uni-tuebingen.de/software/diamond Required version: v0.7.9+
MyTaxa MyTaxa http://enve-omics.ce.gatech.edu/mytaxa The folder must contain the db and utils dirs, and the AllGenomes.faa BLAST database MyTaxa (opt) MyTaxa http://enve-omics.ce.gatech.edu/mytaxa The folder must contain the db and utils dirs, and the AllGenomes.faa.dmnd database
Krona ktImportText https://github.com/marbl/Krona/wiki Krona (opt) ktImportText https://github.com/marbl/Krona/wiki
Barrnap barrnap http://www.vicbioinformatics.com/software.barrnap.shtml
bedtools bedtools http://bedtools.readthedocs.org/en/latest/

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