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updates to READMEs
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cuttlefishh committed Nov 1, 2018
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2 changes: 1 addition & 1 deletion data/README.md
Expand Up @@ -7,7 +7,7 @@ Data files required to run the scripts and notebooks in `code` and generate the
Data files used in Release 1 (Thompson et al., 2017) are found in several locations:

* Most processed files are in the current directory.
* All files except sequences are on the [EMP FTP site](ftp://ftp.microbio.me/emp/release1), with contents listed in `data/ftp_contents.txt`.
* All files except sequences are on the EMP FTP site (ftp://ftp.microbio.me/emp/release1), whose contents are listed in this repository in `data/ftp_contents.txt`.
* All files except sequences are in the [Zenodo archive](https://zenodo.org/record/890000) for the [paper](http://doi.org/10.1038/nature24621).
* Sequences are in the [European Nucleotide Archive](http://ebi.ac.uk/ena/), downloaded using scripts in `code/download-sequences`.

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2 changes: 1 addition & 1 deletion protocols/README.md
Expand Up @@ -4,7 +4,7 @@ This directory contains laboratory protocols and SOPs (or links to them) for sam

### Overview

The first phase of the EMP (2010-2017) explicitly used the DNA extraction and amplicon sequencing protocols, but not necessarily the other protocols, described here. The 16S rRNA amplicon protocol in particular is described in detail by Caporaso et al. ([*PNAS*, 2011](http://doi.org/10.1073/pnas.1000080107)). The full methods used in the meta-analysis of this first phase (Release 1) are described by Thompson et al. ([*Nature*, 2017](http://doi.org/10.1038/nature24621)).
The first phase of the EMP (2010-2017) explicitly used the DNA extraction and amplicon sequencing protocolsbut not necessarily the other protocolsdescribed here. The 16S rRNA amplicon protocol in particular is described in detail by Caporaso et al. ([*PNAS*, 2011](http://doi.org/10.1073/pnas.1000080107)). The full methods used in the meta-analysis of this first phase (Release 1) are described by Thompson et al. ([*Nature*, 2017](http://doi.org/10.1038/nature24621)).

The second phase of the EMP (2016-present), a multi-omics effort informally called "EMP500", uses all of the protocols and SOPs presented here. EMP500 involves the metagenomic sequencing and metabolomic profiling, in addition to amplicon sequencing, of ~500 freshly collected environmental samples from diverse sites on our planet. This multi-omics dataset will be used to address multiple technical and ecological questions in microbial ecology. A biobank of frozen aliquots of samples are being maintained at UCSD and PNNL for future methods testing and analysis.

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