test_beta_diversity.py

#!/usr/bin/env python
# File created on 13 Jul 2012
from __future__ import division

__author__ = "Greg Caporaso"
__copyright__ = "Copyright 2011, The QIIME project"
__credits__ = ["Greg Caporaso", "Jai Ram Rideout"]
__license__ = "GPL"
__version__ = "1.7.0"
__maintainer__ = "Greg Caporaso"
__email__ = "gregcaporaso@gmail.com"
__status__ = "Release"



from glob import glob
from shutil import rmtree
from os.path import exists, join
from cogent.util.unit_test import TestCase, main
from cogent.util.misc import remove_files, create_dir
from cogent.util.unit_test import TestCase, main
from biom.parse import parse_biom_table
from qiime.parse import parse_distmat
from qiime.util import (get_qiime_temp_dir, 
                        get_tmp_filename)
from qiime.test import initiate_timeout, disable_timeout
from qiime.parallel.beta_diversity import (ParallelBetaDiversitySingle,
                               ParallelBetaDiversityMultiple)

class ParallelBetaDiversityTests(TestCase):
    
    def setUp(self):
        
        self.files_to_remove = []
        self.dirs_to_remove = []
        
        # Create example output directory
        tmp_dir = get_qiime_temp_dir()
        self.test_out = get_tmp_filename(tmp_dir=tmp_dir,
                                         prefix='qiime_parallel_tests_',
                                         suffix='',
                                         result_constructor=str)
        self.dirs_to_remove.append(self.test_out)
        create_dir(self.test_out)
        
        self.input1_fp = get_tmp_filename(tmp_dir=self.test_out,
                                            prefix='qiime_inseqs',
                                            suffix='.biom')
        input1_f = open(self.input1_fp,'w')
        input1_f.write(input1)
        input1_f.close()
        self.files_to_remove.append(self.input1_fp)

        self.input2_fp = get_tmp_filename(tmp_dir=self.test_out,
                                            prefix='qiime_inseqs',
                                            suffix='.biom')
        input2_f = open(self.input2_fp,'w')
        input2_f.write(input2)
        input2_f.close()
        self.files_to_remove.append(self.input2_fp)
        
        self.input1_fps = [self.input1_fp,self.input2_fp]
        
        self.tree_fp = get_tmp_filename(tmp_dir=self.test_out,
                                     prefix='qiime',
                                     suffix='.tre')
        tree_f = open(self.tree_fp,'w')
        tree_f.write(tree)
        tree_f.close()
        self.files_to_remove.append(self.tree_fp)
        
        # Define number of seconds a test can run for before timing out 
        # and failing
        initiate_timeout(60)

    
    def tearDown(self):
        
        disable_timeout()
        remove_files(self.files_to_remove)
        # remove directories last, so we don't get errors
        # trying to remove files which may be in the directories
        for d in self.dirs_to_remove:
            if exists(d):
                rmtree(d)

class ParallelBetaDiversitySingleTests(ParallelBetaDiversityTests):
    
    def test_parallel_beta_diversity(self):
        """ parallel beta diveristy functions in single file mode """
        params = {'metrics':'weighted_unifrac,unweighted_unifrac',
                  'tree_path':self.tree_fp,
                  'jobs_to_start':3,
                  'full_tree':False
                  }
        app = ParallelBetaDiversitySingle()
        r = app(self.input1_fp,
                self.test_out,
                params,
                job_prefix='BTEST',
                poll_directly=True,
                suppress_submit_jobs=False)
        input_sample_ids = parse_biom_table(open(self.input1_fp,'U')).SampleIds
        dm_fps = glob(join(self.test_out,'*weighted_unifrac*'))
        for dm_fp in dm_fps:
            dm_sample_ids = parse_distmat(open(dm_fp))[0]
            self.assertEqualItems(dm_sample_ids,input_sample_ids)

    def test_parallel_beta_diversity_wo_tree(self):
        """ parallel beta diveristy functions in single file mode """
        params = {'metrics':'bray_curtis',
                  'tree_path':None,
                  'jobs_to_start':3,
                  'full_tree':False
                  }
        app = ParallelBetaDiversitySingle()
        r = app(self.input1_fp,
                self.test_out,
                params,
                job_prefix='BTEST',
                poll_directly=True,
                suppress_submit_jobs=False)
        input_sample_ids = parse_biom_table(open(self.input1_fp,'U')).SampleIds
        dm_fps = glob(join(self.test_out,'bray_curtis*'))
        for dm_fp in dm_fps:
            dm_sample_ids = parse_distmat(open(dm_fp))[0]
            self.assertEqualItems(dm_sample_ids,input_sample_ids)

class ParallelBetaDiversityMultipleTests(ParallelBetaDiversityTests):

    def test_parallel_beta_diversity(self):
        """ parallel beta diveristy functions in multiple file mode """
        params = {'metrics':'weighted_unifrac',
                  'tree_path':self.tree_fp,
                  'jobs_to_start':3,
                  'full_tree':False
                  }
        app = ParallelBetaDiversityMultiple()
        r = app(self.input1_fps,
                self.test_out,
                params,
                job_prefix='BTEST',
                poll_directly=True,
                suppress_submit_jobs=False)
        input_sample_ids_l = []
        dm_sample_ids_l = []
        for input_fp, dm_fp in zip(self.input1_fps,
                                 glob(join(self.test_out,'*weighted_unifrac*'))):
            input_sample_ids_l.append(list(parse_biom_table(open(input_fp,'U')).SampleIds))
            dm_sample_ids_l.append(parse_distmat(open(dm_fp))[0])
        dm_sample_ids_l.sort()
        input_sample_ids_l.sort()
        self.assertEqual(dm_sample_ids_l,input_sample_ids_l)
        # just confirming that there are different sample ids in
        # the two sets of results
        self.assertNotEqual(dm_sample_ids_l[0],dm_sample_ids_l[1])


    def test_parallel_beta_diversity_wo_tree(self):
        """ parallel beta diveristy functions in multiple file mode without tree 
        """
        params = {'metrics':'bray_curtis',
                  'tree_path':None,
                  'jobs_to_start':3,
                  'full_tree':False
                  }
        app = ParallelBetaDiversityMultiple()
        r = app(self.input1_fps,
                self.test_out,
                params,
                job_prefix='BTEST',
                poll_directly=True,
                suppress_submit_jobs=False)
        input_sample_ids_l = []
        dm_sample_ids_l = []
        for input_fp, dm_fp in zip(self.input1_fps,
                                 glob(join(self.test_out,'*bray_curtis*'))):
            input_sample_ids_l.append(list(parse_biom_table(open(input_fp,'U')).SampleIds))
            dm_sample_ids_l.append(parse_distmat(open(dm_fp))[0])
        dm_sample_ids_l.sort()
        input_sample_ids_l.sort()
        self.assertEqual(dm_sample_ids_l,input_sample_ids_l)
        # just confirming that there are different sample ids in
        # the two sets of results
        self.assertNotEqual(dm_sample_ids_l[0],dm_sample_ids_l[1])


input1 = """{"rows": [{"id": "0", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "1", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Bacilli", "o__Bacillales", "f__Staphylococcaceae"]}}, {"id": "2", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "3", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "4", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "5", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Bacilli", "o__Lactobacillales", "f__Lactobacillaceae"]}}, {"id": "6", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "7", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "8", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "9", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Tenericutes", "c__Erysipelotrichi", "o__Erysipelotrichales", "f__Erysipelotrichaceae"]}}, {"id": "10", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "11", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Ruminococcaceae"]}}, {"id": "12", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "13", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Bacilli", "o__Lactobacillales", "f__Streptococcaceae"]}}, {"id": "14", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Actinobacteria", "c__Actinobacteria", "o__Coriobacteriales", "f__Coriobacteriaceae"]}}, {"id": "15", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "16", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Actinobacteria", "c__Actinobacteria", "o__Coriobacteriales", "f__Coriobacteriaceae"]}}, {"id": "17", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__Porphyromonadaceae"]}}, {"id": "18", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Ruminococcaceae"]}}, {"id": "19", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Ruminococcaceae"]}}, {"id": "20", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "21", "metadata": {"taxonomy": ["Root", "k__Bacteria"]}}, {"id": "22", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Proteobacteria", "c__Deltaproteobacteria", "o__Desulfovibrionales", "f__Desulfovibrionaceae"]}}, {"id": "23", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "24", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "25", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "26", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "27", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__TM7", "c__TM7-3", "o__CW040", "f__F16"]}}, {"id": "28", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "29", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Ruminococcaceae"]}}, {"id": "30", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Ruminococcaceae"]}}, {"id": "31", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "32", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "33", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Actinobacteria", "c__Actinobacteria", "o__Coriobacteriales", "f__Coriobacteriaceae"]}}, {"id": "34", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Actinobacteria", "c__Actinobacteria", "o__Coriobacteriales", "f__Coriobacteriaceae"]}}, {"id": "35", "metadata": {"taxonomy": ["Root", "k__Bacteria"]}}, {"id": "36", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Tenericutes", "c__Erysipelotrichi", "o__Erysipelotrichales", "f__Erysipelotrichaceae"]}}, {"id": "37", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "38", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "39", "metadata": {"taxonomy": ["Root", "k__Bacteria"]}}, {"id": "40", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "41", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Ruminococcaceae"]}}, {"id": "42", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "43", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Ruminococcaceae"]}}, {"id": "44", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "45", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Deferribacteres", "c__Deferribacteres", "o__Deferribacterales", "f__Deferribacteraceae"]}}, {"id": "46", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "47", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "48", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "49", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "50", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "51", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "52", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "53", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "54", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "55", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Tenericutes", "c__Erysipelotrichi", "o__Erysipelotrichales", "f__Erysipelotrichaceae"]}}, {"id": "56", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "57", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "58", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "59", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales"]}}, {"id": "60", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "61", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "62", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "63", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "64", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__Bacteroidaceae"]}}, {"id": "65", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "66", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Ruminococcaceae"]}}, {"id": "67", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "68", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "69", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "70", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "71", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "72", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "73", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Tenericutes", "c__Erysipelotrichi", "o__Erysipelotrichales", "f__Erysipelotrichaceae"]}}, {"id": "74", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "75", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "76", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "77", "metadata": {"taxonomy": ["Root", "k__Bacteria"]}}, {"id": "78", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__Bacteroidaceae"]}}, {"id": "79", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "80", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "81", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Actinobacteria", "c__Actinobacteria", "o__Coriobacteriales", "f__Coriobacteriaceae"]}}, {"id": "82", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__Porphyromonadaceae"]}}, {"id": "83", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "84", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "85", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "86", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "87", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Tenericutes", "c__Mollicutes", "o__RF39", "f__"]}}, {"id": "88", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "89", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "90", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__Porphyromonadaceae"]}}, {"id": "91", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "92", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "93", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "94", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__Porphyromonadaceae"]}}, {"id": "95", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "96", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "97", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "98", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "99", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "100", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", 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{"id": "PC.355", "metadata": null}, {"id": "PC.607", "metadata": null}, {"id": "PC.634", "metadata": null}], "generated_by": "QIIME 1.4.0-dev, svn revision 2835", "matrix_type": "sparse", "shape": [418, 9], "format_url": "http://biom-format.org", "date": "2012-03-14T20:48:29.112457", "type": "OTU table", "id": null, "matrix_element_type": "int"}"""

input2 = """{"rows": [{"id": "0", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "1", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Bacilli", "o__Bacillales", "f__Staphylococcaceae"]}}, {"id": "2", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "3", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "4", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "5", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Bacilli", "o__Lactobacillales", "f__Lactobacillaceae"]}}, {"id": "6", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "7", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "8", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "9", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Tenericutes", "c__Erysipelotrichi", "o__Erysipelotrichales", "f__Erysipelotrichaceae"]}}, {"id": "10", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "11", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Ruminococcaceae"]}}, {"id": "12", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "13", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Bacilli", "o__Lactobacillales", "f__Streptococcaceae"]}}, {"id": "14", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Actinobacteria", "c__Actinobacteria", "o__Coriobacteriales", "f__Coriobacteriaceae"]}}, {"id": "15", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "16", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Actinobacteria", "c__Actinobacteria", "o__Coriobacteriales", "f__Coriobacteriaceae"]}}, {"id": "17", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__Porphyromonadaceae"]}}, {"id": "18", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Ruminococcaceae"]}}, {"id": "19", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Ruminococcaceae"]}}, {"id": "20", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", 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{"id": "36", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Tenericutes", "c__Erysipelotrichi", "o__Erysipelotrichales", "f__Erysipelotrichaceae"]}}, {"id": "37", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "38", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Bacteroidetes", "c__Bacteroidia", "o__Bacteroidales", "f__"]}}, {"id": "39", "metadata": {"taxonomy": ["Root", "k__Bacteria"]}}, {"id": "40", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "41", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Ruminococcaceae"]}}, {"id": "42", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", "c__Clostridia", "o__Clostridiales", "f__Lachnospiraceae"]}}, {"id": "43", "metadata": {"taxonomy": ["Root", "k__Bacteria", "p__Firmicutes", 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tree = 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if __name__ == "__main__":
    main()