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MAFFT.pm
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MAFFT.pm
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# $Id$
#
# BioPerl module for Bio::Tools::Run::Alignment::MAFFT
#
# Cared for by Jason Stajich
#
# Copyright Jason Stajich
#
# You may distribute this module under the same terms as perl itself
#
# POD documentation - main docs before the code
=head1 NAME
Bio::Tools::Run::Alignment::MAFFT - run the MAFFT alignment tools
=head1 SYNOPSIS
use Bio::Tools::Run::Alignment::MAFFT;
=head1 DESCRIPTION
You can get MAFFT from
L<http://www.biophys.kyoto-u.ac.jp/~katoh/programs/align/mafft4/>.
"fftnsi" is the default in this implementation.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/MailList.html - About the mailing lists
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the web:
http://bugzilla.open-bio.org/
=head1 AUTHOR - Jason Stajich
Email jason-at-bioperl.org
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
package Bio::Tools::Run::Alignment::MAFFT;
use vars qw($AUTOLOAD @ISA $PROGRAMNAME $PROGRAM %DEFAULTS
@MAFFT_PARAMS @MAFFT_SWITCHES @OTHER_SWITCHES %OK_FIELD
@MAFFT_ALN_METHODS
);
use strict;
use Bio::Seq;
use Bio::SeqIO;
use Bio::SimpleAlign;
use Bio::AlignIO;
use Bio::Root::Root;
use Bio::Root::IO;
use Bio::Factory::ApplicationFactoryI;
use Bio::Tools::Run::WrapperBase;
@ISA = qw(Bio::Root::Root Bio::Tools::Run::WrapperBase
Bio::Factory::ApplicationFactoryI);
BEGIN {
%DEFAULTS = ( 'OUTPUT' => 'fasta',
'METHOD' => 'fftnsi',
'CYCLES' => 2);
@MAFFT_PARAMS =qw( METHOD CYCLES );
@MAFFT_SWITCHES = qw( NJ ALL_POSITIVE);
@OTHER_SWITCHES = qw(QUIET ALIGN OUTPUT OUTFILE);
@MAFFT_ALN_METHODS = qw(fftnsi fftns nwnsi nwns fftnsrough nwnsrough);
# Authorize attribute fields
foreach my $attr ( @MAFFT_SWITCHES,@MAFFT_PARAMS,@OTHER_SWITCHES ) {
$OK_FIELD{$attr}++;
}
}
=head2 program_name
Title : program_name
Usage : $factory->program_name()
Function: holds the program name
Returns: string
Args : None
=cut
sub program_name {
return 'mafft';
}
=head2 executable
Title : executable
Usage : my $exe = $blastfactory->executable('blastall');
Function: Finds the full path to the 'codeml' executable
Returns : string representing the full path to the exe
Args : [optional] name of executable to set path to
[optional] boolean flag whether or not warn when exe is not found
=cut
sub executable {
my ($self, $exename, $exe,$warn) = @_;
$exename = $self->program_name unless (defined $exename );
if( defined $exe && -x $exe ) {
$self->{'_pathtoexe'}->{$exename} = $exe;
}
unless( defined $self->{'_pathtoexe'}->{$exename} ) {
my $f = $self->program_path($exename);
$exe = $self->{'_pathtoexe'}->{$exename} = $f if(-e $f && -x $f );
# This is how I meant to split up these conditionals --jason
# if exe is null we will execute this (handle the case where
# PROGRAMDIR pointed to something invalid)
unless( $exe ) { # we didn't find it in that last conditional
if( ($exe = $self->io->exists_exe($exename)) && -x $exe ) {
$self->{'_pathtoexe'}->{$exename} = $exe;
} else {
$self->warn("Cannot find executable for $exename") if $warn;
$self->{'_pathtoexe'}->{$exename} = undef;
}
}
}
return $self->{'_pathtoexe'}->{$exename};
}
=head2 program_path
Title : program_path
Usage : my $path = $factory->program_path();
Function: Builds path for executable
Returns : string representing the full path to the exe
Args : none
=cut
sub program_path {
my ($self,$program_name) = @_;
my @path;
push @path, $self->program_dir if $self->program_dir;
push @path, $program_name .($^O =~ /mswin/i ?'.exe':'');
return Bio::Root::IO->catfile(@path);
}
=head2 program_dir
Title : program_dir
Usage : $factory->program_dir(@params)
Function: returns the program directory, obtained from ENV variable.
Returns: string
Args :
=cut
sub program_dir {
return File::Spec->rel2abs($ENV{MAFFTDIR}) if $ENV{MAFFTDIR};
}
sub new {
my ($class,@args) = @_;
my $self = $class->SUPER::new(@args);
my ($attr, $value);
while (@args) {
$attr = shift @args;
$value = shift @args;
next if( $attr =~ /^-/); # don't want named parameters
$self->$attr($value);
}
$self->output($DEFAULTS{'OUTPUT'}) unless( $self->output );
$self->method($DEFAULTS{'METHOD'}) unless( $self->method );
return $self;
}
sub AUTOLOAD {
my $self = shift;
my $attr = $AUTOLOAD;
$attr =~ s/.*:://;
$attr = uc $attr;
# aliasing
$attr = 'OUTFILE' if $attr eq 'OUTFILE_NAME';
$self->throw("Unallowed parameter: $attr !") unless $OK_FIELD{$attr};
$self->{$attr} = shift if @_;
return $self->{$attr};
}
=head2 error_string
Title : error_string
Usage : $obj->error_string($newval)
Function: Where the output from the last analysis run is stored.
Returns : value of error_string
Args : newvalue (optional)
=cut
sub error_string{
my ($self,$value) = @_;
if( defined $value) {
$self->{'error_string'} = $value;
}
return $self->{'error_string'};
}
=head2 version
Title : version
Usage : exit if $prog->version() < 1.8
Function: Determine the version number of the program
Example :
Returns : float or undef
Args : none
=cut
sub version {
my ($self) = @_;
my $exe;
return unless $exe = $self->executable;
# this is a bit of a hack, but MAFFT is just a gawk script
# so we are actually grepping the scriptfile
# UPDATE (Torsten Seemann)
# it now seems to be a 'sh' script and the format has changed
# slightly. i've tried to make the change compatible with both...
# version="v5.860 (2006/06/12)"; export version
if( open(my $NAME, "grep 'export version' $exe | ") ) {
while(<$NAME>) {
if( /version.*?([\d.a-z]+)\s+/ ) {
return $1;
}
}
$self->warn("No version found");
close($NAME);
} else {
$self->warn("$!");
}
return;
}
=head2 run
Title : run
Usage : my $output = $application->run(\@seqs);
Function: Generic run of an application
Returns : Bio::SimpleAlign object
Args : array ref of Bio::PrimarySeqI objects OR
filename of sequences to run with
=cut
sub run {
my ($self,$seqs) = @_;
return $self->align($seqs);
}
=head2 align
Title : align
Usage :
$inputfilename = 't/data/cysprot.fa';
$aln = $factory->align($inputfilename);
or
$seq_array_ref = \@seq_array;
# @seq_array is an array of Seq objs
$aln = $factory->align($seq_array_ref);
Function: Perform a multiple sequence alignment
Returns : Reference to a SimpleAlign object containing the
sequence alignment.
Args : Name of a file containing a set of unaligned fasta sequences
or else an array of references to Bio::Seq objects.
Throws an exception if argument is not either a string (eg a
filename) or a reference to an array of Bio::Seq objects. If
argument is string, throws exception if file corresponding to string
name can not be found. If argument is Bio::Seq array, throws
exception if less than two sequence objects are in array.
=cut
sub align {
my ($self,$input) = @_;
# Create input file pointer
$self->io->_io_cleanup();
my ($infilename,$type) = $self->_setinput($input);
if (! $infilename) {
$self->throw("Bad input data or less than 2 sequences in $input !");
}
my ($param_string,$outstr) = $self->_setparams();
# run mafft
return &_run($self, $infilename, $param_string,$outstr);
}
=head2 _run
Title : _run
Usage : Internal function, not to be called directly
Function: makes actual system call to tcoffee program
Example :
Returns : nothing; tcoffee output is written to a
temporary file OR specified output file
Args : Name of a file containing a set of unaligned fasta sequences
and hash of parameters to be passed to tcoffee
=cut
sub _run {
my ($self,$infilename,$paramstr,$outstr) = @_;
my $commandstring = $self->executable($self->method)." $paramstr $infilename $outstr";
$self->debug( "mafft command = $commandstring \n");
my $status = system($commandstring);
my $outfile = $self->outfile();
if( !-e $outfile || -z $outfile ) {
$self->warn( "MAFFT call crashed: $? [command $commandstring]\n");
return;
}
my $in = Bio::AlignIO->new('-file' => $outfile,
'-format' => $self->output);
my $aln = $in->next_aln();
return $aln;
}
=head2 _setinput
Title : _setinput
Usage : Internal function, not to be called directly
Function: Create input file for mafft programs
Example :
Returns : name of file containing mafft data input
Args : Seq or Align object reference or input file name
=cut
sub _setinput {
my ($self,$input) = @_;
my ($infilename, $seq, $temp, $tfh);
if (! ref $input) {
# check that file exists or throw
$infilename = $input;
unless (-e $input) {return 0;}
return ($infilename);
} elsif (ref($input) =~ /ARRAY/i ) { # $input may be an
# array of BioSeq objects...
# Open temporary file for both reading & writing of array
($tfh,$infilename) = $self->io->tempfile();
if( ! ref($input->[0]) ) {
$self->warn("passed an array ref which did not contain objects to _setinput");
return;
} elsif( $input->[0]->isa('Bio::PrimarySeqI') ) {
$temp = Bio::SeqIO->new('-fh' => $tfh,
'-format' => 'fasta');
my $ct = 1;
foreach $seq (@$input) {
return 0 unless ( ref($seq) &&
$seq->isa("Bio::PrimarySeqI") );
if( ! defined $seq->display_id ||
$seq->display_id =~ /^\s+$/) {
$seq->display_id( "Seq".$ct++);
}
$temp->write_seq($seq);
}
$temp->close();
undef $temp;
close($tfh);
$tfh = undef;
} else {
$self->warn( "got an array ref with 1st entry ".
$input->[0].
" and don't know what to do with it\n");
}
return ($infilename);
} else {
$self->warn("Got $input and don't know what to do with it\n");
}
return 0;
}
=head2 _setparams
Title : _setparams
Usage : Internal function, not to be called directly
Function: Create parameter inputs for mafft program
Example :
Returns : parameter string to be passed to mafft program
Args : name of calling object
=cut
sub _setparams {
my ($self) = @_;
my ($outfile,$param_string) = ('','');
# Set default output file if no explicit output file selected
unless (defined($outfile = $self->outfile) ) {
my $tfh;
($tfh, $outfile) = $self->io->tempfile(-dir=>$self->tempdir());
close($tfh);
undef $tfh;
$self->outfile($outfile);
}
my ($attr,$value);
for $attr ( @MAFFT_SWITCHES) {
$value = $self->$attr();
next unless ($value);
my $attr_key = lc $attr; #put switches in format expected by mafft
$attr_key = ' --'.$attr_key;
$param_string .= $attr_key ;
}
my $method = $self->method;
$self->throw("no method ") unless defined $method;
if( $method !~ /(rough|nsi)$/ &&
defined $self->cycles) {
$param_string .= " ".$self->cycles;
}
my $outputstr = " 1>$outfile" ;
if ($self->quiet() || $self->verbose < 0) {
$param_string .= " --quiet";
$outputstr .= ' 2> /dev/null';
}
return ($param_string, $outputstr);
}
=head2 methods
Title : methods
Usage : my @methods = $self->methods()
Function: Get/Set Alignment methods - NOT VALIDATED
Returns : array of strings
Args : arrayref of strings
=cut
sub methods {
my ($self) = shift;
return @MAFFT_ALN_METHODS;
}
=head1 Bio::Tools::Run::BaseWrapper methods
=cut
=head2 no_param_checks
Title : no_param_checks
Usage : $obj->no_param_checks($newval)
Function: Boolean flag as to whether or not we should
trust the sanity checks for parameter values
Returns : value of no_param_checks
Args : newvalue (optional)
=cut
=head2 save_tempfiles
Title : save_tempfiles
Usage : $obj->save_tempfiles($newval)
Function:
Returns : value of save_tempfiles
Args : newvalue (optional)
=cut
=head2 outfile_name
Title : outfile_name
Usage : my $outfile = $mafft->outfile_name();
Function: Get/Set the name of the output file for this run
(if you wanted to do something special)
Returns : string
Args : [optional] string to set value to
=cut
=head2 tempdir
Title : tempdir
Usage : my $tmpdir = $self->tempdir();
Function: Retrieve a temporary directory name (which is created)
Returns : string which is the name of the temporary directory
Args : none
=cut
=head2 cleanup
Title : cleanup
Usage : $mafft->cleanup();
Function: Will cleanup the tempdir directory
Returns : none
Args : none
=cut
=head2 io
Title : io
Usage : $obj->io($newval)
Function: Gets a L<Bio::Root::IO> object
Returns : L<Bio::Root::IO>
Args : none
=cut
1; # Needed to keep compiler happy