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WOT is a great tool for time coures single cell analysis! thank you for developing it. I want to use it to analyze my reprograming data too, but I was confused about the normalization steps in your parper. It seem that you normalized tha data twice (before and after find HVGs).
I flowed your way (by my own understanding) to use the code below:
WOT is a great tool for time coures single cell analysis! thank you for developing it. I want to use it to analyze my reprograming data too, but I was confused about the normalization steps in your parper. It seem that you normalized tha data twice (before and after find HVGs).
I flowed your way (by my own understanding) to use the code below:
I wondered if the following operation is reasonable:
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