A mock community composed of 48 bacteria represented in equal abundances in three replicate samples. These are the same community members present in mock-2, which consists of the same mock community samples analyzed on a separate Illumina MiSeq run. This was generated by Dr. Sathish Subramanian at Washington University, St. Louis, in 2012.
Previously called dataset-5 in Bokulich et al. 2013 and dataset B1 in Bokulich et al. 2015.
Note:
These barcode reads contain golay barcodes, and the mapping barcodes need to be reverse-complemented to match the reads. Run in qiime-1 using the following command:
split_libraries_fastq.py -i mock-forward-read.fastq.gz -o split_libraries -m sample-metadata.tsv -b mock-index-read.fastq.gz --rev_comp_mapping_barcodes