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README.md
figure1.Rmd
figure1_revision.Rmd
header.tex
runjags_results.Rdata
supplementary.Rmd
supplementary.bib
supplementary.pdf
supplementary_revision.Rmd
supplementary_revision.pdf
treeinform_preprint.pdf

README.md

Revising assemblies with phylogenetic information

This repository contains the code for our manuscript on using phylogenetic information to revise and improve transcriptome assemblies in Agalma1.0. It is described in this preprint:

Guang A, Howison M, Zapata F, Lawrence CE, Dunn CW. Revising transcriptome assemblies with phylogenetic information in Agalma 1.0. bioRxiv 202416; doi: https://doi.org/10.1101/202416

Organization

The repository is organized thusly:

  • treeinform_preprint.pdf: pdf of main manuscript preprint.
  • supplementary.Rmd: R notebook complete with code to generate all the figures and analyses for the Supplementary Information from original manuscript submission.
  • supplementary_revision.Rmd: R notebook complete with code to generate all the figures and analyses for the Supplementary Information from manuscript revision submission.
  • figure1.Rmd: R notebook with code to generate Figure 1 in the original main manuscript submission.
  • figure1_revision.Rmd: R notebook with code to generate Figure 1 in the main manuscript revision submission.
  • data/: folder with all the data that is used in supplementary.Rmd and for Figure 1.
    • revisions/: additional data from our manuscript revision submission
  • code/: folder with all the code that was used to generate data/. This is organized into:
    • agalma/: bash scripts used to run Agalma1.0 on output from a previous genetree run from Agalma1.0, starting from treeinform with different thresholds up to the 2nd genetree run. The sqlite database storing the previous genetree run is not in this repository as it is 3.4GB; if you would like to access the database please email us.
    • cafe/: bash and python scripts used to run CAFE for initial estimates of lambda and mu in our mixture model.
    • phyldog/: bash scripts used to run phyldog on the 2nd genetree run's output from Agalma1.0.
    • revisions/: bash scripts for steps and programs run in our manuscript revision submission, which ran Agalma/1.1 and Trinity/2.5.1
    • functions.R: functions accessed by supplementary.Rmd and supplementary_revision.Rmd.
    • with_internal.tre and species_chron.tre: non-ultrametric and ultrametric tree files used to generate the calibration nodes and times used in supplementary.Rmd. Species_chron.tre was generated with ape::chronos on with_internal.tre.
    • runjags.txt: mixture model structure and parameters specified in JAGS language, for use in runjags in supplementary.Rmd.

Bash scripts for Agalma1.0 and phyldog

All bash scripts were run on the epscor and datasci condos in the Center for Computing and Visualization at Brown inside a conda env. They are written to work with SLURM. If you would like help with running the scripts yourselves or to access the sqlite database the scripts were run on, please email us.