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BAM trimming according to primers with ivar, new rule #113

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michalogit
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The rule is rather simple, works in my internal snakemake on Euler
Fixed the problem I had with iVar, finding the -e switch, which outputs also reads that were not trimmed.
This is not too logical in iVar, compared to other trimming software I've used.
Added also in the same rule samtools sorting and indexing, copying your style from align.smk.

The pull request does not change anything in your files, just adds 3 files...

@BioWu
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BioWu commented Dec 14, 2021

Dose iVar ork well?

@DrYak
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DrYak commented Feb 2, 2022

Related tool that we might want to integrate as well:

Background:

  • since this pull request we created, we have now a mixture of different protocols in our cohort.

@DrYak
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DrYak commented Aug 16, 2022

integrated as of git commit 5154bbb
with working CI/CD

Currently in branch tribble, comming into branches and rubicon

@DrYak DrYak closed this Aug 16, 2022
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3 participants