barcode is 12 bp length

cribbslab · Jul 11, 2023 · 4501d62 · 4501d62
1 parent 295c505
commit 4501d62
Showing 1 changed file with 1 addition and 1 deletion.
diff --git a/tallytrin/python/identify_bcumi_macosko.py b/tallytrin/python/identify_bcumi_macosko.py
@@ -82,7 +82,7 @@ def find_substring(long_string, subset_str):
 
                 bc_start = find_substring(seq, "GTACTCTGCG")
                 if bc_start is not None:
-                    barcode = seq[bc_start-10:bc_start]
+                    barcode = seq[bc_start-12:bc_start]
                     barcodes.append(barcode)
                     umi = seq[bc_start-18:bc_start-12]
                     if umi is None: