Author: @eleni-chr
This toolkit enables morphometric analysis of cells in microscopy images, providing a range of parameters that help researchers understand and study the morphology of cells.
Features
The key feature is the ability to calculate the following parameters from single-channel, single-frame images in TIFF format:
- Cell area
- Cell perimeter
- Cell circularity
- Cell eccentricity (demonstrating the elliptical shape of the cell)
- Cell extent (the ratio of pixels in the object to pixels in the total bounding box)
- Major Axis Length
- Minor Axis Length
- Fractal dimension
- Convex hull area
- Convex hull perimeter
Usage
Run the MATLAB function morphology.m to calculate the parameters listed above. To visually inspect each image and verify the performance of the previous function, use the MATLAB function visInspect.m.
Requirements
This software requires single-channel, single-frame images in TIFF format. Each image should contain a single cell whose full structure is visible. Labelling with phalloidin or a similar substance is one way to achieve this.
Disclaimer: The code provided in this toolkit has not been peer-reviewed and is subject to errors. Users are encouraged to thoroughly test the code and verify its accuracy for their specific use cases. The author of this code is not responsible for any errors or inaccuracies in the results obtained from using these functions. Additionally, the code may be subject to updates or modifications, and users should keep an eye out for future releases to ensure the most accurate and up-to-date functionality.
Full Changelog: https://github.com/eleni-chr/cell-morphometry/commits/v1.0.0
