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A commandline utility to remove 3-prime sequences from next-generation sequencing reads in FastQ formatted files

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erasmus-center-for-biomics/AdapterTrimmer

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Adapter trimmer

A simple trimmer that removes sequences from the 3-prime end of Illumina reads. A simple Make workflow has been added to use this tool on multiple FastQ files at once in the workflow directory.

After building the software with the provided Makefile, 2 programs should be created in the bin directory. These 2 binaries are adapter_trim and iupac_trim. The adapter_trim application removes standard sequences from the reads. The iupac_trim program understands the complete IUPAC nucleotide codes.

The adapter trimmer uses a simple match/mismatch scoring algorithm. Reads are trimmed if more than $match bases match the provided sequence and no more than $mismatch mismatches occurred. Multiple sequences can be processed at once. No specific paired-end functionality is available in this tool, but the first and second reads can be processed independantly.

To do

  • Implement tests software test. Currently, only a test script with simple test data is present.

Installation instruction

Prerequisites:

  • BOOST libraries
  • BOOST development libraries
cd /path/to/AdapterTrimmer/
make ;

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A commandline utility to remove 3-prime sequences from next-generation sequencing reads in FastQ formatted files

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