Skip to content

FusionFlow is a bioinformatic pipeline that enables the detection of gene fusions from RNA and DNA data.

Notifications You must be signed in to change notification settings

federicacitarrella/FusionFlow

Repository files navigation

QUICK START:

1. Install Nextflow and Docker.
     
2. Test the pipeline:
        
        nextflow run federicacitarrella/FusionFlow -profile [test_docker/test_local]

4. Run your own analysis:
        
        nextflow run federicacitarrella/FusionFlow \
                --rnareads “/path/to/rna/reads_{1,2}.*” \ 
                --dnareads_tumor “/path/to/dna/tumor/reads_{3,4}.*” \
                --dnareads_normal “/path/to/dna/normal/reads_{5,6}.*” \
                --arriba --ericscript --fusioncatcher --integrate --genefuse \
                -profile <docker/local>

Before using the local profile you need to create conda virtual environments from the yml files and specify the environment path in the command line (the Arriba path does not need "/bin").
e.g. nextflow run federicacitarrella/FusionFlow \
        --envPath_ericscript /path/to/miniconda3/envs/ericscript/bin \
        --envPath_arriba /path/to/miniconda3/envs/arriba/ \
        --envPath_fusioncatcher /path/to/miniconda3/envs/fusioncatcher/bin \
        --envPath_integrate /path/to/miniconda3/envs/integrate/bin \
        --envPath_genefuse /path/to/miniconda3/envs/genefuse/bin \
         -profile test_local

About

FusionFlow is a bioinformatic pipeline that enables the detection of gene fusions from RNA and DNA data.

Topics

Resources

Stars

Watchers

Forks

Releases

No releases published

Packages

No packages published