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specifically mention guppy5 supper accuracy
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fenderglass committed Sep 5, 2021
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2 changes: 1 addition & 1 deletion README.md
Expand Up @@ -24,7 +24,7 @@ Latest updates

### Flye 2.9 release (20 Aug 2022)
* Better assembly of very short sequences (e.g. plasmids or viruses). They vere often missed in previous versions.
* New --nano-hq mode for ONT Guppy5+ and Q20 reads (3-5% error rate)
* New --nano-hq mode for ONT Guppy5+ (SUP mode) and Q20 reads (3-5% error rate)
* Optimized default parameters for HiFi (HPC error threshold 0.01 -> 0.001; increased min overlap)
* Polishing improvements: reduced number of possible clusters of errors
* Improvements in repeat detection algorithm to further limit a chance of (otherwise infrequent) misassemblies
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2 changes: 1 addition & 1 deletion docs/FAQ.md
Expand Up @@ -50,7 +50,7 @@ Are PacBio CCS/HiFi reads supported?

Yes, use the `--pacbio-hifi` option.

Are there any special parameters/modes for the newest ONT data (Guppy 5+, Q20)?
Are there any special parameters/modes for the newest ONT data (Guppy 5+ SUP, Q20)?
-------------------------------------------------------------------------------

Yes, use the new `--nano-hq` mode.
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2 changes: 1 addition & 1 deletion docs/NEWS.md
@@ -1,7 +1,7 @@
Flye 2.9 release (20 Aug 2022)
=============================
* Better assembly of very short sequences (e.g. plasmids or viruses). They vere often missed in previous versions.
* New --nano-hq mode for ONT Guppy5+ and Q20 reads (3-5% error rate)
* New --nano-hq mode for ONT Guppy5+ SUP and Q20 reads (3-5% error rate)
* Optimized default parameters for HiFi (HPC error threshold 0.01 -> 0.001; increased min overlap)
* Polishing improvements: reduced number of possible clusters of errors
* Improvements in repeat delection algorithm to further limit a chance of (otherwise infrequent) misassemblies
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4 changes: 2 additions & 2 deletions docs/USAGE.md
Expand Up @@ -42,7 +42,7 @@ optional arguments:
--nano-corr path [path ...]
ONT reads that were corrected with other methods (<3% error)
--nano-hq path [path ...]
ONT high-quality reads: Guppy5+ or Q20 (<5% error)
ONT high-quality reads: Guppy5+ SUP or Q20 (<5% error)
--subassemblies path [path ...]
[deprecated] high-quality contigs input
-g size, --genome-size size
Expand Down Expand Up @@ -125,7 +125,7 @@ The dataset was originally released by the
range of datasets, from old R7 pores to the most recent R9.x and R10.x. The
expected error rate is 10-15%.

* For the most recent ONT data basecalled with Guppy5 use the new `--nano-hq` mode.
* For the most recent ONT data basecalled with Guppy5+ SUP use the new `--nano-hq` mode.
Expected error rate is <5%.

* For Q20 data, use a combination of `--nano-hq` and `--read-error 0.03`.
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2 changes: 1 addition & 1 deletion flye/main.py
Expand Up @@ -687,7 +687,7 @@ def check_int_range(value, min_val, max_val, require_odd=False):
help="ONT reads that were corrected with other methods (<3%% error)")
read_group.add_argument("--nano-hq", dest="nano_hq", nargs="+",
default=None, metavar="path",
help="ONT high-quality reads: Guppy5+ or Q20 (<5%% error)")
help="ONT high-quality reads: Guppy5+ SUP or Q20 (<5%% error)")
read_group.add_argument("--subassemblies", dest="subassemblies", nargs="+",
default=None, metavar="path",
help="[deprecated] high-quality contigs input")
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