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how to obtain demultixepled paired-end reads with same structure as input reads, including cell barcodes / UMIs #41

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andreiprodan opened this issue Apr 23, 2024 · 2 comments
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@andreiprodan
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first of all congrats on a great piece of software!

I was wondering whether it's possible to set output options so as to obtain as output FASTQ files with the same structure as the input files (but split per e.g. cell barcode)

E.g. for a 10X 3' library, how to split to per-cell FASTQ so as to keep the structure of the resulting per-cell fastq file pairs the same as the inputs (so cell barcode and UMIs on R1, template on R2). Currently I can only figure out how to get either template reads out, or barcode reads, but not all. The -b, --output-types flag only accepts one value at a time

@nh13
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nh13 commented Apr 23, 2024

Can you try --output-types M T B (space-delimited)?

@nh13 nh13 added the question Further information is requested label Apr 23, 2024
@andreiprodan
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that works, thanks!

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