Indigo is a rapid single-nucleotide variant (SNV) and insertion/deletion (InDel) discovery method in Chromatogram traces obtained from Sanger sequencing of PCR products. It can separate a mutated and wildtype allele and aligns both alleles against a reference sequence or wildtype chromatogram. Indigo discovers mutations generated by genome editing tools such as CRISPR/Cas9 or TALENs.
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Indigo: SNV and InDel Discovery in Chromatogram traces obtained from Sanger sequencing of PCR products https://gear.embl.de/indigo
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